堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)
Mini Samples ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2
- 編號MEA551Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于5.9pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 2360¥ 3372¥ 15174¥ 28662¥ 236040
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-90 | 86 |
| EDTA plasma(n=5) | 80-89 | 86 |
| heparin plasma(n=5) | 95-104 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-101% | 88-95% | 78-94% | 86-98% |
| EDTA plasma(n=5) | 90-102% | 80-95% | 85-92% | 98-105% |
| heparin plasma(n=5) | 89-97% | 96-103% | 80-105% | 91-99% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）25µL，37°C溫育1小時；
3. 吸棄，加檢測溶液A25µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B25µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物25µL，37°C孵育10-20分鐘；
8. 加終止液20µL，立即450nm讀數(shù)。
實驗原理
將堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)與連接于固相載體上的抗體結合，然后加入生物素化的堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA551Hu02 | 堿性成纖維細胞生長因子(FGF2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA551Hu04 | 堿性成纖維細胞生長因子(FGF2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA551Hu01 | 堿性成纖維細胞生長因子(FGF2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA551Hu01 | 堿性成纖維細胞生長因子(FGF2)活性蛋白 | Cell?culture;?Activity?Assays. |
| CPA551Hu21 | 堿性成纖維細胞生長因子(FGF2)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA551Hu01 | 堿性成纖維細胞生長因子(FGF2)多克隆抗體 | WB; IHC; ICC/IF |
| LAA551Hu71 | 堿性成纖維細胞生長因子(FGF2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAA551Hu81 | 堿性成纖維細胞生長因子(FGF2)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAA551Hu22 | 堿性成纖維細胞生長因子(FGF2)單克隆抗體 | Testing in progress |
| MAA551Hu21 | 堿性成纖維細胞生長因子(FGF2)單克隆抗體 | IHC |
| SEA551Hu | 堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA551Hu | 堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA551Hu | 堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA551Hu | 堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA551Hu | 堿性成纖維細胞生長因子(FGF2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA551Hu11 | 堿性成纖維細胞生長因子(FGF2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
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