透明質(zhì)酸(HA)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)
Mini Samples ELISA Kit for Hyaluronic Acid (HA)
Hyaluronan; Hyaluronate
- 編號MEA182Ge
- 物種Pan-species (General，通用)相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長3h
- 檢測范圍4.94-400ng/mL
- 靈敏度最小可檢測劑量小于等于1.77ng/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 4011¥ 5730¥ 25785¥ 48705¥ 401100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
標準曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測透明質(zhì)酸(HA)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的透明質(zhì)酸(HA)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 90-103 | 97 |
| EDTA plasma(n=5) | 78-89 | 84 |
| heparin plasma(n=5) | 80-97 | 80 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的透明質(zhì)酸(HA)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中透明質(zhì)酸(HA)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-104% | 80-92% | 83-92% | 79-98% |
| EDTA plasma(n=5) | 98-105% | 90-101% | 97-105% | 80-94% |
| heparin plasma(n=5) | 82-93% | 78-94% | 90-104% | 80-93% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）25µL后，立即加入檢測溶液A25µL，
37°C孵育1小時；
3. 甩干，洗板3次；
4. 加檢測溶液B50µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物50µL，37°C孵育10－20分鐘；
7. 加終止液25µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質(zhì)水平。將透明質(zhì)酸(HA)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負相關(guān)。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應用(僅供研究使用，不用于臨床診斷！) |
| CPA182Ge11 | 透明質(zhì)酸(HA)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA182Ge21 | 透明質(zhì)酸(HA)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA182Ge01 | 透明質(zhì)酸(HA)多克隆抗體 | ELISA |
| PAA182Ge02 | 透明質(zhì)酸(HA)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA182Ge81 | 透明質(zhì)酸(HA)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAA182Ge71 | 透明質(zhì)酸(HA)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA182Ge22 | 透明質(zhì)酸(HA)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA182Ge21 | 透明質(zhì)酸(HA)單克隆抗體 | ELISA |
| MEA182Ge | 透明質(zhì)酸(HA)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA182Ge | 透明質(zhì)酸(HA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA182Ge | 透明質(zhì)酸(HA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA182Ge11 | 透明質(zhì)酸(HA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Clinical Infectious Diseases | Hemorrhagic Fever Caused by a Novel Bunyavirus in China: Pathogenesis and Correlates of Fatal Outcome[OxfordJournals: source] |
| Critical Care Medicine | Alveolar fluid in acute respiratory distress syndrome promotes fibroblast migration: Role of platelet-derived growth factor pathway*[PubMed: 22713216] |
| European Archives of Oto-Rhino-Laryngology | Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats[Pubmed: 24077847] |
| Journal of Voice | Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study[ScienceDirect: S0892199713002415] |
| Gynecol Oncol. | Resveratrol inhibits ovarian cancer cell adhesion to peritoneal mesothelium in vitro by modulating the production of α5β1 integrins and hyaluronic acid[Pubmed:24995580] |
| Laryngoscope. | Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria[Pubmed:25545625] |
| Life Sci | Pituitary tumor transforming gene as a novel regulatory factor of liver fibrosis[PubMed: 25936962] |
| Nutr Cancer | Camel Milk: Potential Utility as an Adjunctive Therapy to Peg-IFN/RBV in HCV-5 Infected Patients in Egypt[PubMed: 26492131] |
| Journal of Clinical Gastroenterology and Hepatology | Study of Some Serum Biochemical Markers of Liver Fibrosis in Patients with Chronic HCV[ISSN 2575-7733] |
| BMC Complementary and Alternative Medicine | A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritis[pubmed:29166937] |
| Experimental and Therapeutic Medicine | Polycomb group proteins: Novel molecules associated with ultraviolet A-induced photoaging of human skin.[pubmed:28962194] |
| European?Journal of?Applied?Physiology | The effect of aerobic walking and lower body resistance exercise on serum COMP and hyaluronan, in both males and females[Pubmed:29536174] |
| European Food Research and Technology | Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis[10.1007:s00217-018-3119-z] |
| BioMed Research International | Juvenile Ovine Ex Vivo Larynges: Phonatory, Histologic, and Micro CT Based Anatomic Analyses[] |
| Advances in Medical Sciences | Selected elements of extracellular matrix of the skin in diabetes and insulin resistance[Pubmed: 31146169] |
| Viruses | Strain-Dependent Porcine Circovirus Type 2 (PCV2) Entry and Replication in T-Lymphoblasts[Pubmed: 31480752] |
| Physiology International | Betulin attenuated liver damage by prevention of hepatic mitochondrial dysfunction in rats with alcoholic steatohepatitis[Pubmed: 31619044] |
| Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort[Pubmed: 32210109] |
| Journal of Proteomics | iTRAQ-based analysis for the identification of MARCH8 targets in human esophageal squamous cell carcinoma[33540066] |
| Eur J Appl Physiol | An exploratory study to investigate the association between age, physical activity, femoral trochlear cartilage thickness and biomarkers of tissue metabolism in??-[33713200] |
| J Cell Physiol | Trimethylamine N‐oxide exacerbates acetaminophen‐induced liver injury by interfering with macrophage‐mediated liver regeneration[34459512] |
| Experimental Cell Research | Comparative studies on the effect of pig adipose-derived stem cells (pASCs) preconditioned with hypoxia or normoxia on skin wound healing in mice[Pubmed:35718003] |
| Biomedicine & Pharmacotherapy | Bicyclol ameliorates advanced liver diseases in murine models via inhibiting the IL-6/STAT3 signaling pathway[Pubmed:35658240] |
| Sensors and Actuators B: Chemical | Novel multifunctional delivery system for chondrocytes and articular cartilage based on carbon quantum dots[] |
