男人女人午夜视频免费,免费人妻无码不卡中文字幕系,国产A级三级三级三级,成年无码aⅴ片在线观看

干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)

Mini Samples ELISA Kit for Interferon Alpha (IFNa)

IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D

  • 干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)產品包裝(模擬)
  • 干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)產品包裝(模擬)
  • 干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)實驗結果圖
  • MEA033Mu.jpg標準曲線圖
  • Certificate通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,小樣本),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)92-10196
EDTA plasma(n=5)80-9084
heparin plasma(n=5)78-9693

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內加入適量的干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,小樣本),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)78-103%86-94%79-102%89-98%
EDTA plasma(n=5)89-103%79-95%78-101%96-103%
heparin plasma(n=5)87-94%79-90%80-95%97-105%

穩(wěn)定性

經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)25µL,37°C溫育1小時;
3. 吸棄,加檢測溶液A25µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B25µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物25µL,37°C孵育10-20分鐘;
8. 加終止液20µL,立即450nm讀數。

實驗原理

將干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)與連接于固相載體上的抗體結合,然后加入生物素化的干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

相關產品

編號適用物種:Mus musculus (Mouse,小鼠)應用(僅供研究使用,不用于臨床診斷!)
EPA033Mu61干擾素α(IFNa)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA033Mu01干擾素α(IFNa)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA033Mu02干擾素α(IFNa)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA033Mu61干擾素α(IFNa)活性蛋白Cell?culture;?Activity?Assays.
APA033Mu01干擾素α(IFNa)活性蛋白Cell?culture;?Activity?Assays.
PAA033Mu06干擾素α(IFNa)多克隆抗體WB; IHC; ICC; IP.
PAA033Mu01干擾素α(IFNa)多克隆抗體WB; IHC
PAA033Mu02干擾素α(IFNa)多克隆抗體WB; IHC; ICC; IP.
LAA033Mu71干擾素α(IFNa)多克隆抗體(生物素標記)WB; IHC; ICC.
MAA033Mu21干擾素α(IFNa)單克隆抗體WB; IHC; ICC; IP.
MAA033Mu22干擾素α(IFNa)單克隆抗體WB; IHC; ICC; IP.
LAA033Mu72干擾素α(IFNa)單克隆抗體(生物素標記)WB; IHC; ICC.
LAA033Mu76干擾素α(IFNa)單克隆抗體(生物素標記)WB; IHC; ICC.
SEA033Mu干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
MEA033Mu干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,小樣本)Enzyme-linked immunosorbent assay for Antigen Detection.
IEA033Mu干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA033Mu干擾素α(IFNa)檢測試劑盒(化學發(fā)光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMA033Mu干擾素α(IFNa)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA033Mu01干擾素α(IFNa)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志參考文獻
Journal of Animal and Veterinary AdvancesTryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine[Medwell: 2010610616]
Journal of Animal and Veterinary AdvancesImmunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus[Medwell: 2010594600]
Ann Rheum Dis. The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats[PubMed: 23172753]
NanomedicineEfficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform[PubMed: 23394132]
International journal of experimental pathologyExperimental rat model for alcohol-induced osteonecrosis of the femoral head[PubMed: PMC3781777]
Antiviral ResearchA recombinant adenovirus bicistronically expressing porcine interferon-α and interferon-γ enhances antiviral effects against foot-and-mouth disease virus[ScienceDirect: S0166354214000266]
British Journal of PharmacologyAndrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells[Onlinelibrary: bph.12440]
BMC CancerHigh efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model[Pubmed:24950740]
PLoS One.Respiratory Mechanics and Plasma Levels of Tumor Necrosis Factor Alpha and Interleukin 6 Are Affected by Gas Humidification during Mechanical Ventilation in Dogs[Pubmed:25036811]
Veterinary ResearchInfection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain[Pubmed:25106750]
Asian Pac J Trop MedEffect of SecinH3 on lung injury induced by sepsis of rats[PubMed: 26706678]
PLoS?OnesiRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro[Pubmed:26886455]
Plos OneSulforaphane?Suppresses?Hepatitis?C?Virus?Replication?by?Up-ReCavia (Guinea pig )lating?Heme?Oxygenase-1?Expressionthrough?PI3K/Nrf2?Pathway.[Pubmed:27023634]
PLoS One.Vaccination with recombinant Mycobacterium tuberculosis PknD attenuates bacterial dissemination to the brain in Cavia (Guinea pig )inea pigs.[Pubmed:23776655]
Veterinary microbiologyImpact of porcine reproductive and respiratory syndrome virus and porcine circovirus-2 infection on the potency of the classical swine fever vaccine (LOM strain).[pubmed:27599928]
International Journal of?Clinical?and Experimental PathologyHost miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells[files:ijcep0036479.pdf]
Scandinavian?Journal?of?ImmunologySerum?Proteomics?Analysis?in?Rats?of?Immunosuppression?Induced?by?Chronic?Stress.[pubmed:27384769]
Saudi Journal of GastroenterologyDeoxyschizandrin?suppresses?dss-induced?ulcerative?colitis?in?mice.[pubmed:27976641]
Biochemical and Biophysical Research CommunicationsMicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B[pubmed:28342861]
VirologyThe down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection[pubmed:28988058]
Antiviral ResearchCoinjection of a vaccine and anti-viral agents can provide fast-acting protection from foot-and-mouth disease[pubmed:28454913]
Molecular Therapy. Methods & Clinical DevelopmentAnti-high mobility group box 1 antibody ameliorates albuminuria in MRL/lprlupus-prone mice[pubmed:28649578]
Journal of Virology Fucoidan from Fucus vesiculosus suppresses hepatitis B virus replication by enhancing extracellular signal-regulated Kinase activation.[pubmed:28915824]
Clinical?ImmunologyToll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis[Pubmed:30056130]
International?forum?of?allergy?& rhinologyRole of Toll‐like receptor 9 signaling on activation of nasal polyp–derived fibroblasts and its association with nasal polypogenesis[Pubmed:29901280]
LUPUSCollagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosus[Pubmed: 30336754]
Journal of?Pineal?ResearchRole of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice[Pubmed: 30929267]
Veterinary Immunology and ImmunopathologyDifferential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens[]
international immunopharmacologyAdenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in mice[Pubmed: 31228813]
FASEB JournalScavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1[]
Transboundary and Emerging DiseasesPathogenicity of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1–7–4 viruses) in China[Pubmed: 32187856]
archives of virologyA flagellin-adjuvanted inactivated porcine epidemic diarrhea virus (PEDV) vaccine provides enhanced immune protection against PEDV challenge in piglets[Pubmed: 32253616]
AutophagyLDHB inhibition induces mitophagy and facilitates the progression of CSFV infection[Pubmed: 32924761]
THERIOGENOLOGYPorcine soluble CD83 alleviates LPS-induced abortion in mice by promoting Th2 cytokine production, Treg cell generation and trophoblast invasion[Pubmed: 32810792]
Int ImmunopharmacolBNIP3 deletion ameliorated enterovirus 71 infection-induced hand, foot and mouth disease via inhibiting apoptosis, autophagy, and inflammation in mice[Pubmed: 32717566]
Molecular Medicine Reports Aquaporin?4 deletion ameliorates enterovirus 71 infection in mice[Pubmed: 32582978]
Virulence Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon?…[]
РОССИЙСКАЯ СЕЛЬСКОХОЗЯЙСТВЕННАЯ НАУКАГОРМОНАЛЬНЫЙ И ЦИТОКИНОВЫЙ ПРОФИЛЬ КРОВИ МОЛОЧНЫХ КОРОВ В РАННИЙ ПЕРИОД ГЕСТАЦИИ[]
Communications BiologyInfluenza virus neuraminidase regulates host CD8+ T-cell response in mice[33293641]
AnimalsConcentration-Dependent Type 1 Interferon-Induced Regulation of MX1 and FABP3 in Bovine Endometrial Explants[33494350]
Int J DermatolIFN?\|á and TNF?\|á serum levels and their association with disease severity in Egyptian children and adults with alopecia areata[34008204]
Dig Dis SciOrexin A Suppresses the Expression of Exosomal PD-L1 in Colon Cancer and Promotes T Cell Activity by Inhibiting JAK2/STAT3 Signaling Pathway[34097168]
Clinical findings of Talaromyces marneffei infection among patients with anti-interferon-|? immunodeficiency: a prospective cohort study[34144700]
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETYChronic developmental exposure to low-dose ([C8mim][PF6]) induces neurotoxicity and behavioural abnormalities in rats[34555716]
Carbohydrate PolymersThe anti-inflammatory activity of specific-sized hyaluronic acid oligosaccharides[34823813]
Poult Sci Effects of different selenium sources on duodenum and jejunum tight junction network and growth performance of broilers in a model of fluorine-induced?…[Pubmed:35066382]
Veterinary MicrobiologySelf-assembled BPIV3 nanoparticles can induce comprehensive immune responses and protection against BPIV3 challenge by inducing dendritic cell maturation in?…[Pubmed:35395543]