白介素33(IL33)單克隆抗體
Monoclonal Antibody to Interleukin 33 (IL33)
DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules
- 編號(hào)MAB980Ra23
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))C5
- Ig亞型 IgG2a Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPB980Ra01-白介素33(IL33)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Homo sapiens (Human，人)
- 應(yīng)用WB; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 711¥ 1659¥ 2370¥ 5925¥ 23700
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
FITC staining on IF; Sample: Human HepG2 cell; Primary Ab: 30ug/ml Mouse Anti-Rat IL33 Antibody Second Ab: 5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Rat Small intestine lysate Primary Ab: 0.4μg/ml Mouse Anti-Rat IL33 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Samples: Lane1: Rat Lung cells treated with 1μg/ml LPS for 24 hours; Lane2: Untreated Rat Lung lysate;
Primary Ab: 0.2μg/ml Mouse Anti-Rat IL33 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)IL33的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別IL33。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB980Ra01 | 白介素33(IL33)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB980Ra02 | 白介素33(IL33)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB980Ra02 | 白介素33(IL33)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB980Ra01 | 白介素33(IL33)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB980Ra71 | 白介素33(IL33)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB980Ra21 | 白介素33(IL33)單克隆抗體 | WB; IHC |
| MAB980Ra22 | 白介素33(IL33)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB980Ra24 | 白介素33(IL33)單克隆抗體 | WB |
| MAB980Ra23 | 白介素33(IL33)單克隆抗體 | WB; ICC/IF |
| SEB980Ra | 白介素33(IL33)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB980Ra | 白介素33(IL33)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB980Ra | 白介素33(IL33)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| The Korean Journal of Internal Medicine | Interleukin-33, matrix metalloproteinase-9, and tissue ?nhibitor of matrix metalloproteinase-1 in myocardial infarction[PubMed: PMC3604606] |
| Acta Histochem | CXCL13 blockade attenuates lupus nephritis of MRL/lpr mice[PubMed: 26456520] |
| Journal of Interferon & Cytokine Research | Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic Rhinosinusitis[Pubmed:26540312] |
| Journal of Interferon & Cytokine Research | The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway.[pubmed:27348082] |
| Archives of Dermatological Research | IL-33 circulating serum levels are increased in patients with non-segmental generalized vitiligo[pubmed:27388717] |
| Sci Pharm. | Chrysin Protects?Rat?Kidney from Paracetamol-Induced Oxidative Stress, Inflammation, Apoptosis, and Autophagy: A Multi-Biomarker Approach. [pubmed:28134775] |
| Biotechnology & Biotechnological Equipment? | Association of IL-33, IL1RL1 gene polymorphisms with serum IL-33 levels and risk of asthma in adults and asthmatic bronchitis in children (Chinese)[10.1080:13102818.2018.1471361] |
| Tropical Journal of Pharmaceutical Research | Role of IL-33 and ST2 signaling and inflammatory responses in non-small cell lung cancer[172341] |
| Particle and?Fibre?Toxicology | Group II innate lymphoid cells and microvascular dysfunction from pulmonary titanium dioxide nanoparticle exposure[Pubmed: 30413212] |
| Scientific Reports | Interleukin-33 serum levels in postmenopausal women with osteoporosis[Pubmed: 30846811] |
| Cell Reports | Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2s[Pubmed: 32101749] |
| Allergy Asthma & Immunology Research | Relationship of microbial profile with airway immune response in eosinophilic or neutrophilic inflammation of asthmatics[Pubmed: 32141256] |
| Traditional Medicine Research | Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection …[] |
| Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota[] | |
| J Immunol Res | Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus[34395633] |
| Pathogens | Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients[Pubmed:35056005] |
| researchsquare | Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling[] |