角蛋白18(CK18)單克隆抗體
Monoclonal Antibody to Cytokeratin 18 (CK18)
KRT18; CYK18; K18; Keratin 18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18
- 編號(hào)MAB231Hu21
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來(lái)源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))E4
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原RPB231Hu01-角蛋白18(CK18)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/mL
- 且適物種Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC; ICC/IF; FCM
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 634¥ 1478¥ 2112¥ 5280¥ 21120
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Sample: Human Cerebrum Tissue; Primary Ab: 10μg/ml Mouse Anti-Human KRT18 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human KRT18 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Endometrial cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human KRT18 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human KRT18 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human KRT18 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
FITC staining on IF;<br/>Samples: Human HepG2 cell; <br/>Primary Ab: 20μg/ml Mouse Anti-Human KRT18 Antibody<br/>Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu18)
Figure. Western Blot; Sample: Recombinant KRT18, Human.
Western Blot; Sample: Rat Liver lysate
Primary Ab: 3μg/ml Mouse Anti-Human KRT18 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human CK18 Monoclonal Antibody (Catalog MAB231Hu21, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)CK18的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別CK18。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry: 10μg/ml;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB231Hu01 | 角蛋白18(CK18)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB231Hu02 | 角蛋白18(CK18)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB231Hu03 | 角蛋白18(CK18)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB231Hu03 | 角蛋白18(CK18)多克隆抗體 | WB; IHC; FCM |
| PAB231Hu04 | 角蛋白18(CK18)多克隆抗體 | WB |
| PAB231Hu01 | 角蛋白18(CK18)多克隆抗體 | WB; IHC; ICC/IF |
| FAB231Hu03 | 抗角蛋白18(CK18)多克隆抗體 | FCM |
| LAB231Hu81 | 角蛋白18(CK18)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB231Hu71 | 角蛋白18(CK18)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB231Hu27 | 角蛋白18(CK18)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB231Hu24 | 角蛋白18(CK18)單克隆抗體 | WB; ICC/IF; FCM |
| MAB231Hu21 | 角蛋白18(CK18)單克隆抗體 | WB; IHC; ICC/IF; FCM |
| MAB231Hu22 | 角蛋白18(CK18)單克隆抗體 | WB; IHC; ICC/IF; FCM |
| MAB231Hu23 | 角蛋白18(CK18)單克隆抗體 | WB; ICC/IF |
| MAB231Hu25 | 角蛋白18(CK18)單克隆抗體 | WB; IHC; ICC; IP. |
| FAB231Hu02 | 抗角蛋白18(CK18)單克隆抗體 | Flow cytometry. |
| FAB231Hu08 | 抗角蛋白18(CK18)單克隆抗體 | Flow cytometry. |
| FAB231Hu06 | 抗角蛋白18(CK18)單克隆抗體 | Flow cytometry. |
| FAB231Hu04 | 抗角蛋白18(CK18)單克隆抗體 | Flow cytometry. |
| SEB231Hu | 角蛋白18(CK18)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB231Hu | 角蛋白18(CK18)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB231Hu01 | 角蛋白18(CK18)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Can J Physiol Pharmacol.? | Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis[Pubmed:27186801] |
| J Alzheimers Dis | Cigarette Smoke-Induced Alterations in Frontal White Matter Lipid Profiles Demonstrated by MALDI-Imaging Mass Spectrometry: Relevance to Alzheimer's Disease[Pubmed:26836183] |
| bioscience biotechnology research asia | Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring[] |
| Biomed Chromatogr | Lipidomic‐based investigation into the therapeutic effects of PPC and BBD on rats with NAFLD[34727379] |
| J Magn Reson Imaging | Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver?…[Pubmed:35212074] |