死骨片1(SQSTM1)單克隆抗體
Monoclonal Antibody to Sequestosome 1 (SQSTM1)
OSIL; PDB3; ZIP3; p62; p62B; p60; A170; Paget Disease Of Bone 3; Oxidative Stress Induced Like; EBI3-associated protein of 60 kDa; Ubiquitin-binding protein p62
- 編號MAD198Hu22
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C1
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原RPD198Hu01-死骨片1(SQSTM1)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/mL
- 且適物種-
- 應(yīng)用WB; ICC/IF; FCM
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 684¥ 1596¥ 2280¥ 5700¥ 22800
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
FITC staining on IF; Sample: Human Hela cell; Primary Ab: 20μg/ml Mouse Anti-Human SQSTM1 Antibody Second Ab: 5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: Human Placenta lysate Primary Ab: 0.2μg/ml Mouse Anti-Human SQSTM1 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Human A549 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human SQSTM1 Monoclonal Antibody (Catalog MAD198Hu22, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對SQSTM1的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別SQSTM1。
用法
Western blotting: 0.01-2μg/mL
Immunohistochemistry: 2-20μg/mL
Immunocytochemistry: 2-20μg/mL
Flow cytometry: 10μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPD198Hu01 | 死骨片1(SQSTM1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD198Hu01 | 死骨片1(SQSTM1)多克隆抗體 | WB; IHC; ICC/IF; FCM |
| MAD198Hu21 | 死骨片1(SQSTM1)單克隆抗體 | WB; IHC; ICC/IF |
| MAD198Hu24 | 死骨片1(SQSTM1)單克隆抗體 | WB; ICC/IF; FCM |
| MAD198Hu27 | 死骨片1(SQSTM1)單克隆抗體 | WB |
| MAD198Hu22 | 死骨片1(SQSTM1)單克隆抗體 | WB; ICC/IF; FCM |
| MAD198Hu23 | 死骨片1(SQSTM1)單克隆抗體 | WB; ICC/IF |
| MAD198Hu29 | 死骨片1(SQSTM1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD198Hu26 | 死骨片1(SQSTM1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD198Hu25 | 死骨片1(SQSTM1)單克隆抗體 | IHC |
| FAD198Hu08 | 抗死骨片1(SQSTM1)單克隆抗體 | Flow cytometry. |
| FAD198Hu06 | 抗死骨片1(SQSTM1)單克隆抗體 | Flow cytometry. |
| FAD198Hu04 | 抗死骨片1(SQSTM1)單克隆抗體 | Flow cytometry. |
| FAD198Hu02 | 抗死骨片1(SQSTM1)單克隆抗體 | Flow cytometry. |
| SED198Hu | 死骨片1(SQSTM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MED198Hu | 死骨片1(SQSTM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD198Hu | 死骨片1(SQSTM1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| BRIEFINGS IN BIOINFORMATICS | Lysosome activation in peripheral blood mononuclear cells and prognostic significance of circulating LC3B in COVID-19[33620066] |
| BIOMARKERS OF AUTOPHAGY AND ANTIVIRAL ROLE OF PLACENTA FOR PREDICTING PRETERM DELIVERY IN PATIENTS WITH PRETERM DELIVERY??-[] |