沉默調(diào)節(jié)蛋白3(SIRT3)單克隆抗體
Monoclonal Antibody to Sirtuin 3 (SIRT3)
SIR2L3; Silent Mating Type Information Regulation 2 Homolog 3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; SIR2-like protein 3; Regulatory protein SIR2 homolog 3
- 編號MAE913Hu21
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C2
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPE913Hu01-沉默調(diào)節(jié)蛋白3(SIRT3)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 666¥ 1554¥ 2220¥ 5550¥ 22200
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Sample: Human Stomach Tissue; Primary Ab: 30μg/ml Mouse Anti-Human SIRT3 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human SIRT3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human SIRT3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Spleen Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human SIRT3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Figure. Western Blot; Sample: Recombinant SIRT3, Human.
Western Blot; Sample: Rat Skeletal muscle lysate
Primary Ab: 1.5μg/ml Mouse Anti-Human SIRT3 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對SIRT3的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別SIRT3。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APE913Hu01 | 沉默調(diào)節(jié)蛋白3(SIRT3)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPE913Hu01 | 沉默調(diào)節(jié)蛋白3(SIRT3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE913Hu01 | 沉默調(diào)節(jié)蛋白3(SIRT3)多克隆抗體 | WB; IHC; ICC; IP. |
| LAE913Hu81 | 沉默調(diào)節(jié)蛋白3(SIRT3)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAE913Hu71 | 沉默調(diào)節(jié)蛋白3(SIRT3)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAE913Hu21 | 沉默調(diào)節(jié)蛋白3(SIRT3)單克隆抗體 | WB; IHC |
| MAE913Hu22 | 沉默調(diào)節(jié)蛋白3(SIRT3)單克隆抗體 | WB; IHC |
| MAE913Hu23 | 沉默調(diào)節(jié)蛋白3(SIRT3)單克隆抗體 | WB; IHC |
| LAE913Hu42 | 沉默調(diào)節(jié)蛋白3(SIRT3)單克隆抗體(藻紅素標(biāo)記) | WB; IHC; ICC; IF. |
| SEE913Hu | 沉默調(diào)節(jié)蛋白3(SIRT3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME913Hu | 沉默調(diào)節(jié)蛋白3(SIRT3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Toxicology?Letters | SirT3 regulates diabetogenic effects caused by arsenic: An implication for mitochondrial complex II modification[Pubmed: 30385301] |
| International Breastfeeding Journal | Effects of perinatal factors on sirtuin 3, 8-hydroxy-2′-deoxyguanosine, brain-derived neurotrophic factor and serotonin in cord blood and early breast milk: an …[Pubmed: 32552911] |
| Antioxidants | The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of??-[33396247] |