天冬氨酰tRNA合成酶(DARS)多克隆抗體
Polyclonal Antibody to Aspartyl tRNA Synthetase (DARS)
AspRS; PIG40; Aspartate tRNA Ligase 1,Cytoplasmic; Cell proliferation-inducing gene 40 protein
- 編號PAD006Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPD006Hu01-天冬氨酰tRNA合成酶(DARS)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種-
- 應(yīng)用WB; IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human DARS Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human DARS Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Figure. Western Blot; Sample: Recombinant DARS, Human.
Western Blot; Sample: Human Jurkat cell lysate; <br/>Primary Ab: 1μg/ml Rabbit Anti-Human DARS Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: DARS knockout HepG2 cell lysate;
Predicted MW: 46,57kd
Observed MW: 55kd
Primary Ab: 1μg/ml Rabbit Anti-Human DARS Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Serum; <br/>Primary Ab: 1μg/ml Rabbit Anti-Human DARS Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Western Blot; Sample: Human HepG2 cell lysate; <br/>Primary Ab: 1μg/ml Rabbit Anti-Human DARS Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Western Blot; Sample: Human Raji cell lysate; <br/>Primary Ab: 1μg/ml Rabbit Anti-Human DARS Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對DARS的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別DARS。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPD006Hu01 | 天冬氨酰tRNA合成酶(DARS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD006Hu01 | 天冬氨酰tRNA合成酶(DARS)多克隆抗體 | WB; IHC |