甜菜堿高半胱氨酸甲基轉移酶(BHMT)多克隆抗體
Polyclonal Antibody to Betaine Homocysteine Methyltransferase (BHMT)
Betaine—homocysteine S-methyltransferase
- 編號PAG464Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPG464Hu01-甜菜堿高半胱氨酸甲基轉移酶(BHMT)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Mus musculus (Mouse，小鼠), Sus scrofa; Porcine (Pig，豬)
- 應用WB; IHC; FCM
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Porcine Liver Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human BHMT Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Porcine Cardiac Muscle Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human BHMT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Porcine Kidney Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human BHMT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
WB圖
Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human BHMT Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Kidney lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human BHMT Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Liver lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human BHMT Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Kidney lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human BHMT Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human BHMT Polyclonal Antibody (Catalog PAG464Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對BHMT的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別BHMT。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
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相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPG464Hu01 | 甜菜堿高半胱氨酸甲基轉移酶(BHMT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAG464Hu01 | 甜菜堿高半胱氨酸甲基轉移酶(BHMT)多克隆抗體 | WB; IHC; FCM |
| MAG464Hu21 | 甜菜堿高半胱氨酸甲基轉移酶(BHMT)單克隆抗體 | WB; IHC; ICC; IP. |
| SEG464Hu | 甜菜堿高半胱氨酸甲基轉移酶(BHMT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMG464Hu | 甜菜堿高半胱氨酸甲基轉移酶(BHMT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |