真核翻譯延伸因子2(EEF2)多克隆抗體
Polyclonal Antibody to Eukaryotic Translation Elongation Factor 2 (EEF2)
EF2; Polypeptidyl-tRNA Translocase
- 編號(hào)PAC453Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來(lái)源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原RPC453Hu01-真核翻譯延伸因子2(EEF2)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種-
- 應(yīng)用WB; IHC; ICC/IF; FCM
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。 - 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格20μl100μl200μl1ml10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Human Prostate Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human EEF2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human EEF2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human EEF2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Uterine fibroids Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human EEF2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;<br/>Samples: Human HepG2 cell; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human EEF2 Antibody<br/>Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb18)
Figure. Western Blot; Sample: Recombinant EEF2, Human.
Figure. Western Blot; Sample: Lane1: Human Hela Cells; Lane2: Human Jurkat Cells; Lane3: Human HepG2 Cells; Lane4: Human Raji Cells.
Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-human EEF2 Polyclonal Antibody (Catalog # PAC453Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15).Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)EEF2的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別EEF2。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC453Hu01 | 真核翻譯延伸因子2(EEF2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC453Hu01 | 真核翻譯延伸因子2(EEF2)多克隆抗體 | WB; IHC; ICC/IF; FCM |
| FAC453Hu01 | 抗真核翻譯延伸因子2(EEF2)多克隆抗體 | Flow cytometry. |