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60kD熱休克蛋白(Hsp60)多克隆抗體

Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

  • 60kD熱休克蛋白(Hsp60)多克隆抗體產(chǎn)品包裝(模擬)
  • 60kD熱休克蛋白(Hsp60)多克隆抗體產(chǎn)品包裝(模擬)
  • 60kD熱休克蛋白(Hsp60)多克隆抗體DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • 60kD熱休克蛋白(Hsp60)多克隆抗體DAB staining on IHC-P;
    Samples: Human Glioma Tissue;
    Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 60kD熱休克蛋白(Hsp60)多克隆抗體DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 60kD熱休克蛋白(Hsp60)多克隆抗體DAB staining on IHC-P;
    Samples: Human Stomach cancer Tissue;
    Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 60kD熱休克蛋白(Hsp60)多克隆抗體DAB staining on IHC-P;
    Samples: Human Breast cancer Tissue;
    Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 60kD熱休克蛋白(Hsp60)多克隆抗體DAB staining on IHC-P;
    Samples: Human Prostate Tissue;
    Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA822Hu02.jpgWestern Blot; Samples: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Primary Ab: 0.01μg/ml Rabbit Anti-Human Hsp60 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • 60kD熱休克蛋白(Hsp60)多克隆抗體Western Blot; Samples: Lane1: MCF7 cell lysate; Lane2: A549 cell lysate;
    Primary Ab: 0.01μg/ml Rabbit Anti-Human Hsp60 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 60kD熱休克蛋白(Hsp60)多克隆抗體HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human HSPD1 Polyclonal Antibody (Catalog # PAA822Hu02, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • 60kD熱休克蛋白(Hsp60)多克隆抗體Human Hela cell was stained with 20μg/ml rabbit Anti-human Hsp60 Polyclonal Antibody (Catalog PAA822Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

該抗體是針對Hsp60的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別Hsp60。

用法

Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。

贈品

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編號適用物種：Homo sapiens (Human，人)應用(僅供研究使用，不用于臨床診斷！)
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參考文獻

雜志參考文獻
Sci RepHsp60 response in experimental and human temporal lobe epilepsy[PubMed: 25801186]
Cellular?and Molecular Gastroenterology and HepatologySenescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitro[Pubmed: 30878663]
Breast Cancer Res TreatActivation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer[Pubmed: 32601970]