組蛋白H4(H4)多克隆抗體
Polyclonal Antibody to Histone H4 (H4)
- 編號(hào)PAA289Po01
- 物種Sus scrofa; Porcine (Pig，豬)相同的名稱，不同的物種。
- 來(lái)源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原-
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.33mg/mL
- 且適物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠)
- 應(yīng)用WB
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 684¥ 1596¥ 2280¥ 5700¥ 22800
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Western Blot; Sample: Porcine Spleen lysate; Primary Ab: 1μg/ml Rabbit Anti-Porcine H4 Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Thymus lysate;
Primary Ab: 1μg/ml Rabbit Anti-Porcine H4 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Placenta lysate;
Primary Ab: 1μg/ml Rabbit Anti-Porcine H4 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Porcine H4 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)H4的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別H4。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Sus scrofa; Porcine (Pig，豬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| PAA289Po01 | 組蛋白H4(H4)多克隆抗體 | WB |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS One. | N-Acetyl-Heparin Attenuates Acute Lung Injury Caused by Acid Aspiration Mainly by Antagonizing Histones in Mice[Pubmed:24816808] |
| Crit Care. | Impact of plasma histones in human sepsis and their contribution to cellular injury and inflammation[Pubmed:25260379] |
| Biomed Res Int | A Novel Anti-Histone H2 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome[PubMed: 25649891] |
| Journal of Viral Hepatitis | Patients with HBV-related acute-on-chronic liver failure have increased concentrations of extracellular histones aggravating cellular damage and systemic inflammation[pubmed:27660136] |
| Cell?Death?&?Disease | Dexmedetomidine inhibits astrocyte pyroptosis and subsequently protects the brain in in vitro and in vivo models of sepsis[Pubmed: 30778043] |
| Respir Res | Histone H4 induces heparan sulfate degradation by activating heparanase in chlorine gas-induced acute respiratory distress syndrome[Pubmed:35073921] |