嵌套蛋白(NES)多克隆抗體
Polyclonal Antibody to Nestin (NES)
- 編號PAA500Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPA500Mu01-嵌套蛋白(NES)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Homo sapiens (Human，人)
- 應(yīng)用WB; IHC; ICC; IP; FCM
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 583¥ 1361¥ 1944¥ 4860¥ 19440
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
IHC圖
Figure. Western Blot; Sample: Recombinant NES, Mouse.
Western Blot; Sample: SH-SY5Y cell lysate
Primary Ab: 1μg/ml Rabbit Anti-Mouse NES Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Mouse BALB/c 3T3 cells were fixed with 1% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-mouse NES Polyclonal Antibody (Catalog PAA500Mu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對NES的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別NES。
用法
Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA500Mu02 | 嵌套蛋白(NES)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA500Mu01 | 嵌套蛋白(NES)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA500Mu02 | 嵌套蛋白(NES)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA500Mu01 | 嵌套蛋白(NES)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| SEA500Mu | 嵌套蛋白(NES)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA500Mu | 嵌套蛋白(NES)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Kidney?and?Blood?Pressure?Research | Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5[Pubmed:29689548] |
| Neural Plast | P2Y2 Receptor Mediated Neuronal Regeneration and Angiogenesis to Affect Functional Recovery in Rats with Spinal Cord Injury[Pubmed:35154311] |