食欲素A(OXA)多克隆抗體
Polyclonal Antibody to Orexin A (OXA)
Hypocretin-1
- 編號PAA607Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPA607Ra01-食欲素A(OXA)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.42mg/mL
- 且適物種-
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 616¥ 1436¥ 2052¥ 5130¥ 20520
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Sample: Rat Cerebrum Tissue Primary Ab: 10μg/ml Rabbit Anti-Rat OXA Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Figure. Western Blot; Sample: Recombinant OXA, Rat.
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對OXA的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別OXA。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA607Ra01 | 食欲素A(OXA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA607Ra01 | 食欲素A(OXA)多克隆抗體 | WB; IHC; ICC; IP. |
| CEA607Ra | 食欲素A(OXA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA607Ra | 食欲素A(OXA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Frontiers in Neurology | Neuro-Glial and Systemic Mechanisms of Pathological Responses in Rat Models of Primary Blast Overpressure Compared to “Composite” Blast[PubMed: PMC3275793] |
| Neurological Sciences | Pentylenetetrazol-induced seizures are exacerbated by sleep deprivation through orexin receptor-mediated hippocampal cell proliferation[Pubmed: 23852314] |
| Peptides | Orexin-A-induced ERK1/2 activation reverses impaired spatial learning and memory in pentylenetetrazol-kindled rats via OX1R-mediated hippocampal neurogenesis.[Pubmed: 24321199] |
| Alcohol Alcohol | Orexin in Patients with Alcohol Dependence Treated for Relapse Prevention: A Pilot Study[PubMed: 26597795] |
| Indian Journal of Medical Research and Pharmaceutical Sciences | Evaluation of Antidiabetic And Insulintropic Potential of Nigella Sativa Seeds Water Extract and /or Alpha Lipoic Acid in Type 2 Diabetic Rats[Issues%20Pdf: Vol.2] |
| J Clin Res Pediatr Endocninol | Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished Children[PubMed: 26831553] |
| Neural Regen Res | Resuscitation therapy for traumatic brain injury-induced coma in rats: mechanisms of median nerve electrical stimulation[PubMed: 26170820] |
| Mol Med Rep | Wake-promoting actions of median nerve stimulation in TBI-induced coma: An investigation of orexin-A and orexin receptor 1 in the hypothalamic region[PubMed: 26059340] |
| Mol Neurobiol | Application of the Co-culture Membrane System Pointed to a Protective Role of Catestatin on Hippocampal Plus Hypothalamic Neurons Exposed to Oxygen and Glucose Deprivation[article:10.1007] |
| Neural?Regeneration Research | Wake-promoting effects of vagus nerve stimulation after traumatic brain injury: upregulation of orexin-A and orexin receptor type 1 expression in the prefrontal?…[Pubmed:29557373] |
| Journal of Translational Medicine | Recurrent circadian fasting (RCF) improves blood pressure, biomarkers of cardiometabolic risk and regulates inflammation in men[Pubmed: 31426866] |
| iraqi journal of science | Relationship Between Orexin-A and Insulin Resistance in Patients with Type 2 Diabetes Mellitus[] |
| Brain Sci | Changes in the Orexin System in Rats Exhibiting Learned Helplessness Behaviors[34942932] |