維甲酸受體α(RARa)多克隆抗體
Polyclonal Antibody to Retinoic Acid Receptor Alpha (RARa)
RAR-A; NR1B1; RAR; RAR-Alpha; Nuclear Receptor Subfamily 1 Group B Member 1
- 編號PAA976Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPA976Mu01-維甲酸受體α(RARa)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 583¥ 1361¥ 1944¥ 4860¥ 19440
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
IHC圖
AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20μg/ml Rabbit Anti-Mouse RARa Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
AF488 staining on IF;
Sample: MCF7 cell
Primary Ab: 20μg/ml Rabbit Anti-Mouse RARa Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
Figure. Western Blot; Sample: Recombinant RARa, Mouse.
Figure. Western Blot; Sample: Lane1: Human A431 Cells; Lane2: Human HepG2 Cells.
HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-mouse RARa Polyclonal Antibody (Catalog # PAA976Mu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對RARa的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別RARa。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA976Mu01 | 維甲酸受體α(RARa)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA976Mu01 | 維甲酸受體α(RARa)多克隆抗體 | WB; IHC; ICC; IP. |
| FAA976Mu01 | 抗維甲酸受體α(RARa)多克隆抗體 | FCM |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Nature Communications | Retinoic acid receptor alpha is associated with tamoxifen resistance in breast cancer[PubMed: PMC3759040] |
| COPD: Journal of Chronic Obstructive Pulmonary Disease | Retinoic acid receptor alpha: One of plasma biomarkers associated with exacerbation of chronic obstructive pulmonary disease[Pubmed: 24111541] |