Salusin肽β(SALb)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)
Wide-range ELISA Kit for Salusin Beta (SALb)
產品包裝(模擬)
產品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測Salusin肽β(SALb)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的Salusin肽β(SALb)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 94-101 | 97 |
| EDTA plasma(n=5) | 88-95 | 92 |
| heparin plasma(n=5) | 95-103 | 101 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的Salusin肽β(SALb)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中Salusin肽β(SALb)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-103% | 98-105% | 97-105% | 82-103% |
| EDTA plasma(n=5) | 79-97% | 91-103% | 92-101% | 94-101% |
| heparin plasma(n=5) | 82-95% | 90-104% | 95-104% | 85-94% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數。
實驗原理
本試劑盒應用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質水平。將Salusin肽β(SALb)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經洗滌去掉未結合物，然后加入HRP標記的親和素，經過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關，而與樣品中待測物質含量呈負相關。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| CPC026Hu21 | Salusin肽β(SALb)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAC026Hu08 | Salusin肽β(SALb)多克隆抗體 | WB; IHC; ICC; IP. |
| PAC026Hu01 | Salusin肽β(SALb)多克隆抗體 | WB; IHC; ICC; IP. |
| WEC026Hu | Salusin肽β(SALb)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEC026Hu | Salusin肽β(SALb)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC026Hu | Salusin肽β(SALb)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSC026Hu11 | Salusin肽β(SALb)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| Peptides | The bioactive peptides salusins and apelin–36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass[PubMed: 22884920] |
| Department of Medical Biochemistry and Clinical Biochemistry | Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows[ScienceDirect: S0196978113000533] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Association of low maternal levels of salusins with gestational diabetes mellitus and with small-for-gestational-age fetuses.[PubMed: 23178004] |
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| Oxidative Medicine and Cellular Longevity | Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation of Peroxisome Proliferator-Activated Receptor Gamma[10.1155:2017] |
| International Journal Of Cardiology | Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleus[Pubmed: 30581101] |
| Multiple Sclerosis and?Related?Disorders | Increased level of Plasma Salusin-α and Salusin-β in patients with Multiple Sclerosis[Pubmed: 30738876] |
| cardiology in the young | Salusin-α levels are negatively correlated with diastolic blood pressure in children with obesity[Pubmed: 31486354] |
| Redox Biology | Salusin-β mediates tubular cell apoptosis in acute kidney injury: Involvement of the PKC/ROS signaling pathway[Pubmed: 31884071] |
| Journal of international medical research | Salusin-β is superior to salusin-α as a marker for evaluating coronary atherosclerosis[Pubmed: 32054363] |
| cardiovascular drugs and therapy | A TOR2A Gene Product: Salusin-β Contributes to Attenuated Vasodilatation of Spontaneously Hypertensive Rats.[Pubmed: 32458319] |
| Serum level of salusin β as an indicator of metabolic disorders in Acute Lymphoblastic Leucaemia and Wilms Tumour survivors[] | |
| Mol Med Rep | Salusin?β participates in high glucose?induced HK?2 cell ferroptosis in a Nrf?2?dependent manner[34296310] |
