無翅型MMTV整合位點家族成員5A(WNT5A)重組蛋白
Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
- 編號RPP549Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 來源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細(xì)胞定位分泌, 細(xì)胞外基質(zhì)
- 預(yù)測分子量39.5kDa
- 實際分子量40kDa(差異分析請參閱說明書)
- 片段與標(biāo)簽Ile62~Lys380 with N-terminal His Tag
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點8.8
- 應(yīng)用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure. Gene Sequencing (Extract)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APP549Mu01 | 無翅型MMTV整合位點家族成員5A(WNT5A)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPP549Mu01 | 無翅型MMTV整合位點家族成員5A(WNT5A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAP549Mu02 | 無翅型MMTV整合位點家族成員5A(WNT5A)多克隆抗體 | WB; IHC; ICC; IP. |
| PAP549Mu01 | 無翅型MMTV整合位點家族成員5A(WNT5A)多克隆抗體 | WB; IHC; ICC; IP. |
| LAP549Mu71 | 無翅型MMTV整合位點家族成員5A(WNT5A)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAP549Mu81 | 無翅型MMTV整合位點家族成員5A(WNT5A)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAP549Mu21 | 無翅型MMTV整合位點家族成員5A(WNT5A)單克隆抗體 | WB |
| SEP549Mu | 無翅型MMTV整合位點家族成員5A(WNT5A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMP549Mu | 無翅型MMTV整合位點家族成員5A(WNT5A)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSP549Mu01 | 無翅型MMTV整合位點家族成員5A(WNT5A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Liver International | Secreted frizzled-related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis[Pubmed:25488180] |
| Journal of Physiology and Biochemistry | Wnt5a reverses the inhibitory effect of hyperoxia on transdifferentiation of alveolar epithelial type II cells to type I cells[PubMed: 26547443] |
| Liver | Secreted frizzled‐related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis[PubMed: 25488180] |
| Transl Res | Wnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell cultures[PubMed: 26163991] |
| 不確定雜志名 | Untersuchungen der Serum-Konzentrationen des proinflammatorischen Glykoproteins wnt-5a und seines antiinflammatorischen Inhibitors sFRP-5 bei an …[servlets:MCRFileNodeServlet] |
| Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways.[pubmed:28754618] |
| Arteriosclerosis, Thrombosis, and?Vascular Biology? | Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With?…[Pubmed:29674475] |
| Stem?Cells | Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis‐Supportive Function of Fanconi Anemia Mesenchymal Stromal Cells[Pubmed:29377497] |
| Science Translational Medicine | Adipose tissue–derived WNT5A regulates vascular redox signaling in obesity via USP17/RAC1-mediated activation of NADPH oxidases[Pubmed: 31534019] |
| J Clin Endocrinol Metab | Role of wnt5a in Metabolic Inflammation in Humans[Pubmed: 30137542] |
| Cancer Research | Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis[Pubmed: 31932454] |
| CLINICAL BIOCHEMISTRY | Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease[Pubmed: 33188773] |
| Sao Paulo Medical Journal | Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and …[Pubmed: 32491086] |
| Biomaterials | An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties[34823885] |
| PLoS One | Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study[Pubmed:34990470] |
| J Clin Periodontol | Association between bone turnover markers and periodontitis: A population‐based cross‐sectional study[Pubmed:35569034] |