抵抗素(RETN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Resistin (RETN)
ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3
- 編號KSA847Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.056ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 5378¥ 8964¥ 16135¥ 31374¥ 53784
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對抵抗素(RETN)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| EPA847Hu61 | 抵抗素(RETN)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA847Hu01 | 抵抗素(RETN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA847Hu61 | 抵抗素(RETN)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA847Hu06 | 抵抗素(RETN)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA847Hu01 | 抵抗素(RETN)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA847Hu81 | 抵抗素(RETN)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAA847Hu71 | 抵抗素(RETN)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA847Hu27 | 抵抗素(RETN)單克隆抗體 | IHC |
| MAA847Hu29 | 抵抗素(RETN)單克隆抗體 | IHC |
| MAA847Hu23 | 抵抗素(RETN)單克隆抗體 | WB; IHC |
| MAA847Hu21 | 抵抗素(RETN)單克隆抗體 | IHC |
| MAA847Hu24 | 抵抗素(RETN)單克隆抗體 | IHC |
| MAA847Hu25 | 抵抗素(RETN)單克隆抗體 | IHC |
| MAA847Hu22 | 抵抗素(RETN)單克隆抗體 | WB; IHC; ICC; IP. |
| LAA847Hu82 | 抵抗素(RETN)單克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAA847Hu72 | 抵抗素(RETN)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEA847Hu | 抵抗素(RETN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA847Hu | 抵抗素(RETN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA847Hu | 抵抗素(RETN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA847Hu01 | 抵抗素(RETN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| European Journal of Pharmacology | Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet[PubMed: 20869961] |
| Evid Based Complement Alternat Med. | Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats[Pubmed:Pmc4363645] |
| J Endocrinol. | Estradiol stimulates mitochondrial biogenesis and adiponectin expression in skeletal muscle[Pubmed:24681828] |
| Life Sciences | Adipose tissue regulates hepatic cholesterol metabolism via adiponectin[Pubmed:25445438] |
| Obesity Surgery | Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats[Pubmed:25697126] |
| Evid Based Complement Alternat Med | Tinospora crispa[PubMed: 25821506] |
| Journal of | Longitudinal changes in adipose tissue of dairy cows from late pregnancy to lactation. Part 1: The adipokines apelin and resistin and their relationship to receptors linked with lipolysis[PubMed: 26686707] |
| Genome | Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 3 Diabetic Patients With and Without Complications[PubMed: 25120108] |
| Acta Histochemica | Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines[science:S0065128116301234] |
| Journal of Dairy Science | Endocrine activity of adipose tissues as influenced by energy intake in the periparturient cow[108495429X:34] |
| Evidence-Based?Complementary and Alternative?Medicine | Four-Week?Consumption?of?Malaysian?Honey?Reduces?Excess?Weight?Gainand?Improves?Obesity-Related?Parameters?in High Fat Diet?Induced?ObeseRats.[pubmed:28246535] |
| Oncotarget | FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathway[pubmed:28515350] |
| Journal of Dairy Science | Involvement of plasma adipokines in metabolic and reproductive parameters in Holstein dairy cows fed with diets with differing energy levels[pubmed:28803009] |
| Toxicology | Biological effects of adipocytes in sulfur mustard induced toxicity[pubmed:29129815] |
| Breast?Cancer?Research | Bashar Zeidan1, Antigoni Manousopoulou2, Diana J. Garay-Baquero2, 3, Cory H. White3, 7, Samantha ET Larkin1, Kathleen N. Potter1, Theodoros I?…[Pubmed:29566726] |
| Bioscience Reports | Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats[Pubmed:29784871] |
| Oncotarget | Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1?…[Pubmed:29484120] |
| Journal of Functional Foods | Mango leaf tea promotes hepatoprotective effects in obese rats[Doi: 10.1016/j.jff.2018.09.010] |
| Food?Research?International | Bacupari (Garcinia brasiliensis) extract modulates intestinal microbiota and reduces oxidative stress and inflammation in obese rats[Doi: 10.1016/j.foodres.2019.04.012] |
| Journal of Functional Foods | Extruded sorghum (Sorghum bicolor L.) improves gut microbiota, reduces inflammation, and oxidative stress in obese rats fed a high-fat diet[] |
| Nat Commun | Antidiabetic and cardiovascular beneficial effects of a liver-localized mitochondrial uncoupler[Pubmed: 31092829] |
| Nutrients | Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of?…[Pubmed: 31514318] |
| GYNECOLOGICAL ENDOCRINOLOGY | Saliva resistin as a screening marker of gestational diabetes mellitus[Pubmed: 32804001] |
| Chinese Journal of Natural Medicines | Tao-Hong-Si-Wu Decoction promotes angiogenesis after cerebral ischaemia in rats via platelet microparticles[Pubmed: 32768169] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body …[Pubmed: 32545900] |
| Seasonal and Nutritional Fluctuations in the mRNA Levels of the Short Form of the Leptin Receptor (LRa) in the Hypothalamus and Anterior Pituitary in Resistin?…[34438908] | |
| High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients[] | |
| Skeletal Muscle Fatty Acid Metabolism is Diverted Towards the Synthesis of Signaling Intermediates Without Fatty Acid Uptake Contribution in Dry Cows with Obesity?…[] |