抵抗素(RETN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Resistin (RETN)
ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3
- 編號SEA847Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.625-40ng/mL
- 靈敏度最小可檢測劑量小于等于0.251ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 2092¥ 2988¥ 13446¥ 25398¥ 209160
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測抵抗素(RETN)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的抵抗素(RETN)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 92-104 | 101 |
| EDTA plasma(n=5) | 96-105 | 102 |
| heparin plasma(n=5) | 91-101 | 96 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的抵抗素(RETN)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中抵抗素(RETN)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-99% | 81-104% | 86-97% | 83-101% |
| EDTA plasma(n=5) | 79-95% | 94-103% | 81-103% | 91-98% |
| heparin plasma(n=5) | 79-96% | 87-98% | 86-94% | 84-99% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將抵抗素(RETN)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的抵抗素(RETN)與連接于固相載體上的抗體結合，然后加入生物素化的抵抗素(RETN)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的抵抗素(RETN)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| EPA847Hu61 | 抵抗素(RETN)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA847Hu01 | 抵抗素(RETN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA847Hu61 | 抵抗素(RETN)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA847Hu06 | 抵抗素(RETN)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA847Hu01 | 抵抗素(RETN)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA847Hu81 | 抵抗素(RETN)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAA847Hu71 | 抵抗素(RETN)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA847Hu27 | 抵抗素(RETN)單克隆抗體 | IHC |
| MAA847Hu29 | 抵抗素(RETN)單克隆抗體 | IHC |
| MAA847Hu23 | 抵抗素(RETN)單克隆抗體 | WB; IHC |
| MAA847Hu21 | 抵抗素(RETN)單克隆抗體 | IHC |
| MAA847Hu24 | 抵抗素(RETN)單克隆抗體 | IHC |
| MAA847Hu25 | 抵抗素(RETN)單克隆抗體 | IHC |
| MAA847Hu22 | 抵抗素(RETN)單克隆抗體 | WB; IHC; ICC; IP. |
| LAA847Hu82 | 抵抗素(RETN)單克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAA847Hu72 | 抵抗素(RETN)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEA847Hu | 抵抗素(RETN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA847Hu | 抵抗素(RETN)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA847Hu | 抵抗素(RETN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA847Hu01 | 抵抗素(RETN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
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| Evid Based Complement Alternat Med | Tinospora crispa[PubMed: 25821506] |
| Journal of | Longitudinal changes in adipose tissue of dairy cows from late pregnancy to lactation. Part 1: The adipokines apelin and resistin and their relationship to receptors linked with lipolysis[PubMed: 26686707] |
| Genome | Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 3 Diabetic Patients With and Without Complications[PubMed: 25120108] |
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| Bioscience Reports | Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats[Pubmed:29784871] |
| Oncotarget | Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1?…[Pubmed:29484120] |
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| Food?Research?International | Bacupari (Garcinia brasiliensis) extract modulates intestinal microbiota and reduces oxidative stress and inflammation in obese rats[Doi: 10.1016/j.foodres.2019.04.012] |
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| Nat Commun | Antidiabetic and cardiovascular beneficial effects of a liver-localized mitochondrial uncoupler[Pubmed: 31092829] |
| Nutrients | Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of?…[Pubmed: 31514318] |
| GYNECOLOGICAL ENDOCRINOLOGY | Saliva resistin as a screening marker of gestational diabetes mellitus[Pubmed: 32804001] |
| Chinese Journal of Natural Medicines | Tao-Hong-Si-Wu Decoction promotes angiogenesis after cerebral ischaemia in rats via platelet microparticles[Pubmed: 32768169] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body …[Pubmed: 32545900] |
| Seasonal and Nutritional Fluctuations in the mRNA Levels of the Short Form of the Leptin Receptor (LRa) in the Hypothalamus and Anterior Pituitary in Resistin?…[34438908] | |
| High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients[] | |
| Skeletal Muscle Fatty Acid Metabolism is Diverted Towards the Synthesis of Signaling Intermediates Without Fatty Acid Uptake Contribution in Dry Cows with Obesity?…[] |
