沉默調節(jié)蛋白1(SIRT1)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Sirtuin 1 (SIRT1)
SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment
- 編號KSE912Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍0.78-50ng/mL
- 可適靈敏度0.28ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價格¥ 7560¥ 12600¥ 22680¥ 44100¥ 75600
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產品包裝(模擬)
產品包裝(模擬)
通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對沉默調節(jié)蛋白1(SIRT1)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPE912Hu01 | 沉默調節(jié)蛋白1(SIRT1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE912Hu01 | 沉默調節(jié)蛋白1(SIRT1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAE912Hu81 | 沉默調節(jié)蛋白1(SIRT1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAE912Hu71 | 沉默調節(jié)蛋白1(SIRT1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAE912Hu22 | 沉默調節(jié)蛋白1(SIRT1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAE912Hu23 | 沉默調節(jié)蛋白1(SIRT1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAE912Hu21 | 沉默調節(jié)蛋白1(SIRT1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEE912Hu | 沉默調節(jié)蛋白1(SIRT1)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME912Hu | 沉默調節(jié)蛋白1(SIRT1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSE912Hu01 | 沉默調節(jié)蛋白1(SIRT1)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSE912Hu01 | 沉默調節(jié)蛋白1(SIRT1)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| PLoS One. | SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular Diseases[Plos: Source] |
| PLOS ONE | A Novel Role for SIRT-1 in L-Arginine Protection against STZ Induced Myocardial Fibrosis in Rats[Pubmed:25501750] |
| Journal of Alzheimer's Disease | Serum miR-206 and miR-132 as Potential Circulating Biomarkers for Mild Cognitive Impairment[Pubmed:25589731] |
| Respirology | Increases in peripheral SIRT1: A new biological characteristic of asthma[PubMed: 26040995] |
| PLOS ONE | A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly:[PubMed: 25785999] |
| Endocrine | Changes of serum Mir-217 and the correlation with the severity in type 2 diabetes patients with different stages of diabetic kidney disease.[pubmed:27522360] |
| Naunyn Schmiedebergs Arch Pharmacol | The potential curative effect of rebamipide in hepatic ischemia/reperfusion injury[pubmed:28361247] |
| ?International journal of cardiology | A sirtuin 1/MMP2 prognostic index for myocardial infarction in patients with advanced coronary artery disease[pubmed:28043667] |
| International Journal of Cardiology | Serum concentrations and gene expression of sirtuin 1 in healthy and slightly overweight subjects after caloric restriction or resveratrol supplementation: A randomized trial[pubmed:28029409] |
| Hepatology International | Association of SIRT1 gene polymorphism and its expression for the risk of alcoholic fatty liver disease in the Han population[pubmed:29189974] |
| Toxicology and Applied Pharmacology | Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1)[pubmed:28974454] |
| Chemico-biological Interactions | Magnolol protects pancreatic β-cells against methylglyoxal-induced cellular dysfunction[pubmed:28919305] |
| Nutrients | Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after?…[Pubmed:30037068] |
| Experimental Gerontology | Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia[Pubmed:30071285] |
| Kidney?and?Blood?Pressure?Research | The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy[Pubmed: 30504713] |
| Journal of International Medical Research | Serum and follicular fluid levels of sirtuin 1, sirtuin 6, and resveratrol in women undergoing fertilization: an observational, clinical study[Pubmed: 30556451] |
| BioMed Research International | Centella asiatica Prevents Increase of Hippocampal Tumor Necrosis Factor-Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic?…[] |
| 學位論文 | Influência do tratamento da doen?a periodontal na sirtuína 1 e na lectina ligadora de manose em indivíduos com doen?a arterial coronária[] |
| Journal?of?Physics?D: Applied?Physics | Premature Myocardial Infarction: Genetic Variations in SIRT1 Affect Disease Susceptibility[] |
| Journal of Geriatric?Oncology | Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors[Pubmed: 30952517] |
| Biomed?Research International | Prevents Increase of Hippocampal Tumor Necrosis Factor-α Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of?…[Pubmed: 30956976] |
| Journal of Diabetes and its Complications | Vasohibin-1 in T2DM with different UACR[] |
| Clinical Interventions in Aging | The Serum Concentration of Anti-Aging Proteins, Sirtuin1 and αKlotho in Patients with End-Stage Kidney Disease on Maintenance Hemodialysis[Pubmed: 32214805] |
| Compr Psychiatry | Crocin alleviates schizophrenia-like symptoms in rats by upregulating silent information regulator-1 and brain derived neurotrophic factor[Pubmed: 33045669] |
| Сибирский федеральный университет | Влияние гречневой крупы на сиртуиновую активность у подопытных животных в эксперименте[] |
| Journal of Cereal Science | Dietary buckwheat enhances sirtuin1 without calorie restriction[] |
| Clin Interv Aging | Molecular Changes in Circulating microRNAs' Expression and Oxidative Stress in Adults with Mild Cognitive Impairment: A Biochemical and Molecular Study[33447019] |
| Antioxidants | The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of??-[33396247] |
| Int Urol Nephrol | Evaluation of sirtuin 1 (SIRT1) levels in autosomal dominant polycystic kidney disease[33864594] |
| Neurochem Int | Dimethyl fumarate abridged tauo-/amyloidopathy in a D-Galactose/ovariectomy-induced Alzheimer's-like disease: Modulation of AMPK/SIRT-1, AKT/CREB/BDNF, AKT ?-[34052296] |
| Toxicol Appl Pharmacol | Prasugrel anti-ischemic effect in rats: Modulation of hippocampal SUMO2/3-IкBα/Ubc9 and SIRT-1/miR-22 trajectories[34174262] |
| Food Chem | Buckwheat-enriched diet alleviates bisphenol A mediated oxidative stress via modulation of sirtuin 1 and antioxidant status in experimental rats[34763933] |
| Life Sci | Novel mechanistic insights towards the repositioning of alogliptin in Parkinson's disease[34774622] |
| Int J Mol Sci | Intralesional Infiltrations of Arteriosclerotic Tissue Cells-Free Filtrate Reproduce Vascular Pathology in Healthy Recipient Rats[Pubmed:35163435] |
| J Clin Med | Effects of Coffee on Sirtuin-1, Homocysteine, and Cholesterol of Healthy Adults: Does the Coffee Powder Matter?[Pubmed:35683374] |
| Cell & Bioscience | OGT Protects Skeletal Muscle From Damage Through SIRT1 O-GlcNAcylation During Cold Stress[] |