沉默調(diào)節(jié)蛋白1(SIRT1)重組蛋白
Recombinant Sirtuin 1 (SIRT1)
SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment
- 編號(hào)RPE912Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來(lái)源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位細(xì)胞核
- 預(yù)測(cè)分子量30.3kDa
- 實(shí)際分子量19&28kDa(差異分析請(qǐng)參閱說(shuō)明書)
- 片段與標(biāo)簽Glu244~Ala498 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)5.9
- 應(yīng)用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請(qǐng)參見活性蛋白。 - 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格10μg50μg200μg1mg5mg
- 價(jià)格¥ 1200¥ 3000¥ 6000¥ 18000¥ 45000
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure. Gene Sequencing (Extract)
Figure. SDS-PAGE
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPE912Hu01 | 沉默調(diào)節(jié)蛋白1(SIRT1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE912Hu01 | 沉默調(diào)節(jié)蛋白1(SIRT1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAE912Hu81 | 沉默調(diào)節(jié)蛋白1(SIRT1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAE912Hu71 | 沉默調(diào)節(jié)蛋白1(SIRT1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAE912Hu22 | 沉默調(diào)節(jié)蛋白1(SIRT1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAE912Hu23 | 沉默調(diào)節(jié)蛋白1(SIRT1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAE912Hu21 | 沉默調(diào)節(jié)蛋白1(SIRT1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEE912Hu | 沉默調(diào)節(jié)蛋白1(SIRT1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME912Hu | 沉默調(diào)節(jié)蛋白1(SIRT1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSE912Hu01 | 沉默調(diào)節(jié)蛋白1(SIRT1)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSE912Hu01 | 沉默調(diào)節(jié)蛋白1(SIRT1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS One. | SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular Diseases[Plos: Source] |
| PLOS ONE | A Novel Role for SIRT-1 in L-Arginine Protection against STZ Induced Myocardial Fibrosis in Rats[Pubmed:25501750] |
| Journal of Alzheimer's Disease | Serum miR-206 and miR-132 as Potential Circulating Biomarkers for Mild Cognitive Impairment[Pubmed:25589731] |
| Respirology | Increases in peripheral SIRT1: A new biological characteristic of asthma[PubMed: 26040995] |
| PLOS ONE | A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly:[PubMed: 25785999] |
| Endocrine | Changes of serum Mir-217 and the correlation with the severity in type 2 diabetes patients with different stages of diabetic kidney disease.[pubmed:27522360] |
| Naunyn Schmiedebergs Arch Pharmacol | The potential curative effect of rebamipide in hepatic ischemia/reperfusion injury[pubmed:28361247] |
| ?International journal of cardiology | A sirtuin 1/MMP2 prognostic index for myocardial infarction in patients with advanced coronary artery disease[pubmed:28043667] |
| International Journal of Cardiology | Serum concentrations and gene expression of sirtuin 1 in healthy and slightly overweight subjects after caloric restriction or resveratrol supplementation: A randomized trial[pubmed:28029409] |
| Hepatology International | Association of SIRT1 gene polymorphism and its expression for the risk of alcoholic fatty liver disease in the Han population[pubmed:29189974] |
| Toxicology and Applied Pharmacology | Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1)[pubmed:28974454] |
| Chemico-biological Interactions | Magnolol protects pancreatic β-cells against methylglyoxal-induced cellular dysfunction[pubmed:28919305] |
| Nutrients | Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after?…[Pubmed:30037068] |
| Experimental Gerontology | Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia[Pubmed:30071285] |
| Kidney?and?Blood?Pressure?Research | The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy[Pubmed: 30504713] |
| Journal of International Medical Research | Serum and follicular fluid levels of sirtuin 1, sirtuin 6, and resveratrol in women undergoing fertilization: an observational, clinical study[Pubmed: 30556451] |
| BioMed Research International | Centella asiatica Prevents Increase of Hippocampal Tumor Necrosis Factor-Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic?…[] |
| 學(xué)位論文 | Influência do tratamento da doen?a periodontal na sirtuína 1 e na lectina ligadora de manose em indivíduos com doen?a arterial coronária[] |
| Journal?of?Physics?D: Applied?Physics | Premature Myocardial Infarction: Genetic Variations in SIRT1 Affect Disease Susceptibility[] |
| Journal of Geriatric?Oncology | Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors[Pubmed: 30952517] |
| Biomed?Research International | Prevents Increase of Hippocampal Tumor Necrosis Factor-α Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of?…[Pubmed: 30956976] |
| Journal of Diabetes and its Complications | Vasohibin-1 in T2DM with different UACR[] |
| Clinical Interventions in Aging | The Serum Concentration of Anti-Aging Proteins, Sirtuin1 and αKlotho in Patients with End-Stage Kidney Disease on Maintenance Hemodialysis[Pubmed: 32214805] |
| Compr Psychiatry | Crocin alleviates schizophrenia-like symptoms in rats by upregulating silent information regulator-1 and brain derived neurotrophic factor[Pubmed: 33045669] |
| Сибирский федеральный университет | Влияние гречневой крупы на сиртуиновую активность у подопытных животных в эксперименте[] |
| Journal of Cereal Science | Dietary buckwheat enhances sirtuin1 without calorie restriction[] |
| Clin Interv Aging | Molecular Changes in Circulating microRNAs' Expression and Oxidative Stress in Adults with Mild Cognitive Impairment: A Biochemical and Molecular Study[33447019] |
| Antioxidants | The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of??-[33396247] |
| Int Urol Nephrol | Evaluation of sirtuin 1 (SIRT1) levels in autosomal dominant polycystic kidney disease[33864594] |
| Neurochem Int | Dimethyl fumarate abridged tauo-/amyloidopathy in a D-Galactose/ovariectomy-induced Alzheimer's-like disease: Modulation of AMPK/SIRT-1, AKT/CREB/BDNF, AKT ?-[34052296] |
| Toxicol Appl Pharmacol | Prasugrel anti-ischemic effect in rats: Modulation of hippocampal SUMO2/3-IкBα/Ubc9 and SIRT-1/miR-22 trajectories[34174262] |
| Food Chem | Buckwheat-enriched diet alleviates bisphenol A mediated oxidative stress via modulation of sirtuin 1 and antioxidant status in experimental rats[34763933] |
| Life Sci | Novel mechanistic insights towards the repositioning of alogliptin in Parkinson's disease[34774622] |
| Int J Mol Sci | Intralesional Infiltrations of Arteriosclerotic Tissue Cells-Free Filtrate Reproduce Vascular Pathology in Healthy Recipient Rats[Pubmed:35163435] |
| J Clin Med | Effects of Coffee on Sirtuin-1, Homocysteine, and Cholesterol of Healthy Adults: Does the Coffee Powder Matter?[Pubmed:35683374] |
| Cell & Bioscience | OGT Protects Skeletal Muscle From Damage Through SIRT1 O-GlcNAcylation During Cold Stress[] |