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表皮生長因子受體2(ErbB2)活性蛋白

Active Receptor Tyrosine Protein Kinase erbB-2 (ErbB2)

CD340; EGFR2; HER-2/neu; HER2; NEU; MLN 19; NGL; TKR1; p185; Epidermal Growth Factor Receptor 2; V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2; Neuro/Glioblastoma Derived Oncogene Homolog

  • 表皮生長因子受體2(ErbB2)活性蛋白產(chǎn)品包裝(模擬)
  • 表皮生長因子受體2(ErbB2)活性蛋白產(chǎn)品包裝(模擬)
  • APB867Hu01.pngFigure. SDS-PAGE
  • 表皮生長因子受體2(ErbB2)活性蛋白Figure. Western Blot
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

活性實驗

Figure. The binding activity of EGRF2 with ACTb.
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands. Epidermal growth factor receptor 2 (EGFR2) is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. The EGFR is essential for ductal development of the mammary glands, and agonists of the EGFR such as amphiregulin, TGF-α, and heregulin induce both ductal and lobuloalveolar development even in the absence of estrogen and progesterone. Besides, Actin Beta (ACTb) has been identified as an interactor of EGFR2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human EGFR2 and recombinant human ACTb. Briefly, EGFR2 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-EGFR2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of EGFR2 and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。

相關(guān)產(chǎn)品

編號適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPB867Hu01表皮生長因子受體2(ErbB2)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APB867Hu02表皮生長因子受體2(ErbB2)活性蛋白Cell?culture;?Activity?Assays.
APB867Hu01表皮生長因子受體2(ErbB2)活性蛋白Cell?culture;?Activity?Assays.
RPB867Hu02表皮生長因子受體2(ErbB2)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAB867Hu02表皮生長因子受體2(ErbB2)多克隆抗體WB
PAB867Hu01表皮生長因子受體2(ErbB2)多克隆抗體WB
LAB867Hu81表皮生長因子受體2(ErbB2)多克隆抗體(異硫氰酸熒光素標記)WB; IHC; ICC; IF.
LAB867Hu71表皮生長因子受體2(ErbB2)多克隆抗體(生物素標記)WB; IHC; ICC.
MAB867Hu24表皮生長因子受體2(ErbB2)單克隆抗體WB; IHC; ICC; IP.
MAB867Hu27表皮生長因子受體2(ErbB2)單克隆抗體WB; IHC; ICC; IP.
MAB867Hu29表皮生長因子受體2(ErbB2)單克隆抗體WB; IHC; ICC; IP.
MAB867Hu23表皮生長因子受體2(ErbB2)單克隆抗體ICC/IF
MAB867Hu21表皮生長因子受體2(ErbB2)單克隆抗體WB; IHC; ICC; IP.
MAB867Hu22表皮生長因子受體2(ErbB2)單克隆抗體WB; IHC; ICC; IP.
SEB867Hu表皮生長因子受體2(ErbB2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB867Hu表皮生長因子受體2(ErbB2)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.

參考文獻

雜志參考文獻
Neuroscience LetterTherapeutic effect of co-transplantation of neuregulin 1-transfected-Schwann cells and bone marrow stromal cells on spinal cord hemisection syndrom[Cyagen: source]
Journal of Electroanalytical ChemistryA highly sensitive label-free electrochemical immunosensor based on AuNPs-PtNPs-MOFs for nuclear matrix protein 22 analysis in urine sample[]