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Gremlin 1蛋白(GREM1)活性蛋白

Active Gremlin 1 (GREM1)

PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1

  • Gremlin 1蛋白(GREM1)活性蛋白產(chǎn)品包裝(模擬)
  • Gremlin 1蛋白(GREM1)活性蛋白產(chǎn)品包裝(模擬)
  • APC128Ra01.pngFigure. SDS-PAGE
  • Gremlin 1蛋白(GREM1)活性蛋白Figure. Western Blot
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Figure 1. Cell proliferation of MCF-7 cells after stimulated with GREM1.
Gremlin(GREM1) is an inhibitor in the TGF beta signaling pathway. GREM1and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. A proliferation assay was conducted to detect the bioactivity of recombinant rat GREM1 using A549 cells. Briefly,?A549 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM??prior to the addition of various concentrations of GREM1. After incubated for 96h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly,10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C. Proliferation of A549 cells after incubation with GREM1 for 96h observed by inverted microscope was shown in Figure1. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with recombinant GREM1 for 96h. The result was shown in Figure2. It was obvious that GREM1 significantly increased cell viability of A549 cells.(A)MCF-7 cells cultured in DMEM, stimulated with 0.001ng/ml GREM1 for 96h; (B)Unstimulated MCF7 cells cultured in DMEM for 96h.

Figure. Cell proliferation of MCF-7 cells after stimulated with GREM1.

Gremlin (GREM1) is an inhibitor in the TGF beta signaling pathway. GREM1 and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. It is reported that some BMP target genes is antagonised by GREM1 and GREM2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GREM1 and recombinant human BMP2. Briefly, GREM1 was diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to BMP2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GREM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450 nm immediately. The binding activity of GREM1 and BMP2 was shown in Figure 3, the EC50?for this effect is 1.042 ug/mL.

用法

Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

相關(guān)產(chǎn)品

編號(hào)適用物種:Rattus norvegicus (Rat,大鼠)應(yīng)用(僅供研究使用,不用于臨床診斷!)
APC128Ra01Gremlin 1蛋白(GREM1)活性蛋白Cell?culture;?Activity?Assays.
RPC128Ra01Gremlin 1蛋白(GREM1)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAC128Ra01Gremlin 1蛋白(GREM1)多克隆抗體WB
SEC128RaGremlin 1蛋白(GREM1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMC128RaGremlin 1蛋白(GREM1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
PGC128Ra01Gremlin 1蛋白(GREM1)引物對(duì)PCR

參考文獻(xiàn)

雜志參考文獻(xiàn)
LungIntrinsic BMP Antagonist Gremlin-1 as a Novel Circulating Marker in Pulmonary Arterial Hypertension[PubMed: 25926293]
Tumour BiolOverexpression of Gremlin promotes non-small cell lung cancer progression[PubMed: 26392110]
Int J CardiolTriple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-term prognosis in suspected and proven acute heart failure patients[PubMed: 26539964]
International Journal of CardiologyTriple?head-to-head?comparison?of?fibrotic?biomarkers?galectin-3,?osteopontin?and?gremlin-1?for?long-termprognosis?in?suspected?and?proven?acute?heart?failure?patients[Pubmed:26539964]
American Journal of Respiratory Cell and Molecular BiologyBMPR2 Mutation-independent Mechanisms of Disrupted BMP Signaling in IPAH[Pubmed:27187737]
J RheumatolGREM1 Is a Key ReCavia (Guinea pig )lator of Synoviocyte Hyperplasia and Invasiveness[Pubmed:26834210]
European cells & materialsThe BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells.[pubmed:28266688]
Sci Rep.Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients.[pubmed:28327551]
American Journal of Respiratory Cell and Molecular BiologyBone Morphogenic Protein Type 2 Receptor Mutation-Independent Mechanisms of Disrupted Bone Morphogenetic Protein Signaling in Idiopathic Pulmonary Arterial …[rcmb.2015-0402OC]
Molecular Medicine ReportsGremlin-1 suppression increases BMP-2-induced osteogenesis of human mesenchymal stem cells[pubmed:28260028]
Cellular Haemostasis and PlateletsPlatelets as a novel source of Gremlin-1: Implications for thrombo inflammation[pubmed:27929199]
Scientific ReportsGremlin, A Potential Urinary Biomarker of Anca-Associated Crescentic Glomerulonephritis[Pubmed: 31053735]
International Journal of Molecular SciencesTranscriptomic and Proteomic Analyses Reveal the Potential Mode of Action of Chondrocyte Sheets in Hyaline Cartilage Regeneration[Pubmed: 31878307]
American Journal of Physiology-Cell PhysiologySuppression of microRNA-23a/b-3p delays the development degenerative joint disease by activating Grem1 and TGF-β/Smad signaling pathway[Pubmed: 32186934]
InflammopharmacologyProtective role of microRNA-23a/b-3p inhibition against osteoarthritis through Gremlin1-depenent activation of TGF-β/smad signaling in chondrocytesa[Pubmed:35441352]
Mol CarcinogCAF‐derived exosomes transmitted Gremlin‐1 promotes cancer progression and decreases the sensitivity of hepatoma cells to sorafenib[Pubmed:35638711]