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Gremlin 1蛋白(GREM1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit for Gremlin 1 (GREM1)

PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1

  • Gremlin 1蛋白(GREM1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Gremlin 1蛋白(GREM1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Gremlin 1蛋白(GREM1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)實(shí)驗(yàn)結(jié)果圖
  • SEC128Ra.jpg標(biāo)準(zhǔn)曲線圖
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)Gremlin 1蛋白(GREM1),經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制,不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè),因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的Gremlin 1蛋白(GREM1)(加標(biāo)樣品),重復(fù)測(cè)定并計(jì)算其均值,回收率為測(cè)定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)78-9986
EDTA plasma(n=5)87-10290
heparin plasma(n=5)88-9591

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定,每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的Gremlin 1蛋白(GREM1),并倍比稀釋成1:2,1:4,1:8,1:16的待測(cè)樣本,線性范圍即為稀釋后樣本中Gremlin 1蛋白(GREM1)含量的測(cè)定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)82-93%91-99%87-102%81-102%
EDTA plasma(n=5)91-105%83-91%85-98%96-105%
heparin plasma(n=5)83-97%82-94%83-97%90-98%

穩(wěn)定性

經(jīng)測(cè)定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品及樣本)100µL,37°C孵育1小時(shí);
3. 吸棄,加檢測(cè)溶液A100µL,37°C孵育1小時(shí);
4. 洗板3次;
5. 加檢測(cè)溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將Gremlin 1蛋白(GREM1)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本,其中的Gremlin 1蛋白(GREM1)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的Gremlin 1蛋白(GREM1)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標(biāo)記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的Gremlin 1蛋白(GREM1)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值),計(jì)算樣品濃度。

相關(guān)產(chǎn)品

編號(hào)適用物種:Rattus norvegicus (Rat,大鼠)應(yīng)用(僅供研究使用,不用于臨床診斷!)
APC128Ra01Gremlin 1蛋白(GREM1)活性蛋白Cell?culture;?Activity?Assays.
RPC128Ra01Gremlin 1蛋白(GREM1)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAC128Ra01Gremlin 1蛋白(GREM1)多克隆抗體WB
SEC128RaGremlin 1蛋白(GREM1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMC128RaGremlin 1蛋白(GREM1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
PGC128Ra01Gremlin 1蛋白(GREM1)引物對(duì)PCR

參考文獻(xiàn)

雜志參考文獻(xiàn)
LungIntrinsic BMP Antagonist Gremlin-1 as a Novel Circulating Marker in Pulmonary Arterial Hypertension[PubMed: 25926293]
Tumour BiolOverexpression of Gremlin promotes non-small cell lung cancer progression[PubMed: 26392110]
Int J CardiolTriple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-term prognosis in suspected and proven acute heart failure patients[PubMed: 26539964]
International Journal of CardiologyTriple?head-to-head?comparison?of?fibrotic?biomarkers?galectin-3,?osteopontin?and?gremlin-1?for?long-termprognosis?in?suspected?and?proven?acute?heart?failure?patients[Pubmed:26539964]
American Journal of Respiratory Cell and Molecular BiologyBMPR2 Mutation-independent Mechanisms of Disrupted BMP Signaling in IPAH[Pubmed:27187737]
J RheumatolGREM1 Is a Key ReCavia (Guinea pig )lator of Synoviocyte Hyperplasia and Invasiveness[Pubmed:26834210]
European cells & materialsThe BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells.[pubmed:28266688]
Sci Rep.Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients.[pubmed:28327551]
American Journal of Respiratory Cell and Molecular BiologyBone Morphogenic Protein Type 2 Receptor Mutation-Independent Mechanisms of Disrupted Bone Morphogenetic Protein Signaling in Idiopathic Pulmonary Arterial …[rcmb.2015-0402OC]
Molecular Medicine ReportsGremlin-1 suppression increases BMP-2-induced osteogenesis of human mesenchymal stem cells[pubmed:28260028]
Cellular Haemostasis and PlateletsPlatelets as a novel source of Gremlin-1: Implications for thrombo inflammation[pubmed:27929199]
Scientific ReportsGremlin, A Potential Urinary Biomarker of Anca-Associated Crescentic Glomerulonephritis[Pubmed: 31053735]
International Journal of Molecular SciencesTranscriptomic and Proteomic Analyses Reveal the Potential Mode of Action of Chondrocyte Sheets in Hyaline Cartilage Regeneration[Pubmed: 31878307]
American Journal of Physiology-Cell PhysiologySuppression of microRNA-23a/b-3p delays the development degenerative joint disease by activating Grem1 and TGF-β/Smad signaling pathway[Pubmed: 32186934]
InflammopharmacologyProtective role of microRNA-23a/b-3p inhibition against osteoarthritis through Gremlin1-depenent activation of TGF-β/smad signaling in chondrocytesa[Pubmed:35441352]
Mol CarcinogCAF‐derived exosomes transmitted Gremlin‐1 promotes cancer progression and decreases the sensitivity of hepatoma cells to sorafenib[Pubmed:35638711]