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腫瘤蛋白p53(P53)活性蛋白

Active Tumor Protein p53 (P53)

TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

  • 腫瘤蛋白p53(P53)活性蛋白產(chǎn)品包裝(模擬)
  • 腫瘤蛋白p53(P53)活性蛋白產(chǎn)品包裝(模擬)
  • APA928Ra01.pngFigure. SDS-PAGE
  • 腫瘤蛋白p53(P53)活性蛋白Figure. Western Blot
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Tumor protein p53 (TP53), also known as p53, cellular tumor antigen p53 has many mechanisms of anticancer function and plays a role in apoptosis, genomic stability, and inhibition of angiogenesis. TP53 act as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It also Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. Besides, CREB Binding Protein (CREBBP) has been identified as an interactor of TP53, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TP53 and recombinant rat CREBBP. Briefly, TP53 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to CREBBP-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TP53 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TP53 and CREBBP was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of TP53 with CREBBP.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

相關(guān)產(chǎn)品

編號(hào)適用物種:Rattus norvegicus (Rat,大鼠)應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA928Ra01腫瘤蛋白p53(P53)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA928Ra01腫瘤蛋白p53(P53)活性蛋白Cell?culture;?Activity?Assays.
PAA928Ra01腫瘤蛋白p53(P53)多克隆抗體WB,ICC/IF
MAA928Ra22腫瘤蛋白p53(P53)單克隆抗體WB; ICC/IF
MAA928Ra21腫瘤蛋白p53(P53)單克隆抗體ICC/IF
SEA928Ra腫瘤蛋白p53(P53)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA928Ra腫瘤蛋白p53(P53)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志參考文獻(xiàn)
Anticancer ResearchAnalysis of p53 and miRNA expression after irradiation of glioblastoma cell lines[PubMed: 23155233]
Microscopy Research and TechniqueTherapeutic role of curcumin in oxidative DNA damage caused by formaldehyde[PubMed: 25761397]
J Nutr BiochemSupplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing mice[PubMed: 26344777]
Biochemical and Biophysical Research CommunicationsTranscription factor HBP1: A regulator of senescence and apoptosis of preadipocytes[Pubmed: 31331641]
Folia BiologicaEffect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats[]
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?…[]