腫瘤蛋白p53(P53)單克隆抗體
Monoclonal Antibody to Tumor Protein p53 (P53)
TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
- 編號MAA928Ra22
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)D10
- Ig亞型 IgG2a lambda
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPA928Ra01-腫瘤蛋白p53(P53)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.45mg/ml
- 且適物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Chicken (Gallus，雞)
- 應(yīng)用WB; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 659¥ 1537¥ 2196¥ 5490¥ 21960
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
FITC staining on IF; Sample: Human HepG2 cell; Primary Ab: 40μg/ml Mouse Anti-Rat TP53 Antibody Second Ab: 5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
Western Blot; Sample: Lane1: Rat Colon lysate; Lane2: A431 cell lysate Primary Ab: 0.5ug/ml Mouse Anti-Rat P53 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: Porcine Colon lysate; Lane2: Porcine Spleen lysate; Lane3: Porcine Mammary gland lysate; Lane4: Porcine Skin lysate
Primary Ab: 0.5ug/ml Mouse Anti-Rat P53 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: 3T3-L1 cell lysate; Lane2: RAW264.7 cell lysate; Lane3: Canine Colon lysate; Lane4: Gallus Small intestine lysate; Lane5: Bovine Colon lysate; Lane6: Caprine Colon lysate
Primary Ab: 0.5ug/ml Mouse Anti-Rat P53 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對P53的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別P53。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-40μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA928Ra01 | 腫瘤蛋白p53(P53)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA928Ra01 | 腫瘤蛋白p53(P53)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA928Ra01 | 腫瘤蛋白p53(P53)多克隆抗體 | WB,ICC/IF |
| MAA928Ra22 | 腫瘤蛋白p53(P53)單克隆抗體 | WB; ICC/IF |
| MAA928Ra21 | 腫瘤蛋白p53(P53)單克隆抗體 | ICC/IF |
| SEA928Ra | 腫瘤蛋白p53(P53)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA928Ra | 腫瘤蛋白p53(P53)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Anticancer Research | Analysis of p53 and miRNA expression after irradiation of glioblastoma cell lines[PubMed: 23155233] |
| Microscopy Research and Technique | Therapeutic role of curcumin in oxidative DNA damage caused by formaldehyde[PubMed: 25761397] |
| J Nutr Biochem | Supplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing mice[PubMed: 26344777] |
| Biochemical and Biophysical Research Communications | Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytes[Pubmed: 31331641] |
| Folia Biologica | Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats[] |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?…[] |