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腫瘤蛋白p53(P53)多克隆抗體

Polyclonal Antibody to Tumor Protein p53 (P53)

TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

  • 腫瘤蛋白p53(P53)多克隆抗體產品包裝(模擬)
  • 腫瘤蛋白p53(P53)多克隆抗體產品包裝(模擬)
  • 腫瘤蛋白p53(P53)多克隆抗體FITC staining on IF; Samples: Human HepG2 cell; Primary Ab: 20μg/ml Rabbit Anti-Human TP53 Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
  • 腫瘤蛋白p53(P53)多克隆抗體DAB staining on IHC-P;<br/>Sample: Porcine Colon Tissue<br/>Primary Ab: 10μg/ml Rabbit Anti-Human P53 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
  • 腫瘤蛋白p53(P53)多克隆抗體DAB staining on IHC-P;<br/>Sample: Porcine Lymph node Tissue<br/>Primary Ab: 10μg/ml Rabbit Anti-Human P53 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
  • PAA928Hu01.jpgFigure 1. Western Blot; Sample: Recombinant TP53, Human.
  • 腫瘤蛋白p53(P53)多克隆抗體Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Human 293T cell lysate<br/>Primary Ab: 1μg/mL Rabbit Anti-Human TP53 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
  • 腫瘤蛋白p53(P53)多克隆抗體Knockout Varification: <br/>Lane 1: Wild-type MCF7 cell lysate; <br/>Lane 2: TP53 knockout MCF7 cell lysate; <br/>Predicted MW: 24,30,33,34,38,39,44kDa <br/>Observed MW: 50kDa<br/>Primary Ab: 1μg/ml Rabbit Anti-Human TP53 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
  • 腫瘤蛋白p53(P53)多克隆抗體Western Blot; Sample: Lane1: A431 cell lysate; Lane2: MCF7 cell lysate<br/>Primary Ab: 0.3μg/ml Rabbit Anti-Human TP53 Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
  • 腫瘤蛋白p53(P53)多克隆抗體Western Blot; Sample: Porcine Skin lysate<br/>Primary Ab: 0.3μg/ml Rabbit Anti-Human TP53 Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
  • Certificate通過ISO 9001、ISO 13485質量體系認證

特異性

該抗體是針對P53的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別P53。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當的條件下存儲,損失率低于5%。

贈品

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編號適用物種:Homo sapiens (Human,人)應用(僅供研究使用,不用于臨床診斷!)
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參考文獻

雜志參考文獻
Anticancer ResearchAnalysis of p53 and miRNA expression after irradiation of glioblastoma cell lines[PubMed: 23155233]
Microscopy Research and TechniqueTherapeutic role of curcumin in oxidative DNA damage caused by formaldehyde[PubMed: 25761397]
J Nutr BiochemSupplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing mice[PubMed: 26344777]
Biochemical and Biophysical Research CommunicationsTranscription factor HBP1: A regulator of senescence and apoptosis of preadipocytes[Pubmed: 31331641]
Folia BiologicaEffect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats[]
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?…[]