腫瘤蛋白p53(P53)多克隆抗體
Polyclonal Antibody to Tumor Protein p53 (P53)
TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
- 編號(hào)PAA928Hu02
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來(lái)源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原RPA928Hu02-腫瘤蛋白p53(P53)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種-
- 應(yīng)用WB; IHC; FCM
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。 - 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 589¥ 1373¥ 1962¥ 4905¥ 19620
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Sample: Human Colorectal cancer Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human P53 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human P53 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human P53 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Stomach cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human P53 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human P53 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human P53 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: 293T cell lysate; Lane2: A431 cell lysate Primary Ab: 0.03μg/ml Rabbit Anti-Human P53 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Human Hacat cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human P53 Polyclonal Antibody (Catalog PAA928Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)P53的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別P53。
用法
Western blottin:g: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA928Hu01 | 腫瘤蛋白p53(P53)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA928Hu02 | 腫瘤蛋白p53(P53)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA928Hu03 | 腫瘤蛋白p53(P53)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA928Hu01 | 腫瘤蛋白p53(P53)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA928Hu01 | 腫瘤蛋白p53(P53)多克隆抗體 | WB; IHC; ICC/IF |
| PAA928Hu02 | 腫瘤蛋白p53(P53)多克隆抗體 | WB; IHC; FCM |
| LAA928Hu71 | 腫瘤蛋白p53(P53)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA928Hu22 | 腫瘤蛋白p53(P53)單克隆抗體 | WB |
| MAA928Hu24 | 腫瘤蛋白p53(P53)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA928Hu25 | 腫瘤蛋白p53(P53)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA928Hu27 | 腫瘤蛋白p53(P53)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA928Hu21 | 腫瘤蛋白p53(P53)單克隆抗體 | WB |
| MAA928Hu23 | 腫瘤蛋白p53(P53)單克隆抗體 | WB |
| FAA928Hu04 | 抗腫瘤蛋白p53(P53)單克隆抗體 | Flow cytometry. |
| FAA928Hu02 | 抗腫瘤蛋白p53(P53)單克隆抗體 | Flow cytometry. |
| SEA928Hu | 腫瘤蛋白p53(P53)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA928Hu | 腫瘤蛋白p53(P53)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA928Hu | 腫瘤蛋白p53(P53)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA928Hu01 | 腫瘤蛋白p53(P53)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Anticancer Research | Analysis of p53 and miRNA expression after irradiation of glioblastoma cell lines[PubMed: 23155233] |
| Microscopy Research and Technique | Therapeutic role of curcumin in oxidative DNA damage caused by formaldehyde[PubMed: 25761397] |
| J Nutr Biochem | Supplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing mice[PubMed: 26344777] |
| Biochemical and Biophysical Research Communications | Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytes[Pubmed: 31331641] |
| Folia Biologica | Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats[] |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?…[] |