視黃醇結(jié)合蛋白4(RBP4)多克隆抗體
Polyclonal Antibody to Retinol Binding Protein 4 (RBP4)
PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma
- 編號(hào)PAA929Bo01
- 物種Bos taurus; Bovine (Cattle，牛)相同的名稱(chēng)，不同的物種。
- 來(lái)源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類(lèi)型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原RPA929Bo01-視黃醇結(jié)合蛋白4(RBP4)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Sus scrofa; Porcine (Pig，豬), Capra hircus; Caprine (Goat，山羊)
- 應(yīng)用WB,IHC
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 680¥ 1588¥ 2268¥ 5670¥ 22680
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Sample: Porcine Pancreas Tissue Primary Ab: 10μg/ml Rabbit Anti-Bovine RBP4 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 10μg/ml Rabbit Anti-Bovine RBP4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Bovine Liver Tissue
Primary Ab: 20μg/ml Rabbit Anti-Bovine RBP4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Bovine Kidney Tissue
Primary Ab: 20μg/ml Rabbit Anti-Bovine RBP4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Liver Tissue
Primary Ab: 10μg/ml Rabbit Anti-Bovine RBP4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Figure. Western Blot; Sample: Recombinant RBP4, Bovine.
Knockout Varification: <br/>Lane 1: Wild-type HepG2 cell lysate; <br/>Lane 2: RBP4 knockout HepG2 cell lysate; <br/>Predicted MW: 21kDa <br/>Observed MW: 21kDa<br/>Primary Ab: 1μg/ml Rabbit Anti-Bovine RBP4 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Western Blot; Sample: Bovine Kidney lysate;
Primary Ab: 1μg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Bovine Serum;
Primary Ab: 1μg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Caprine Plasma;
Primary Ab: 1μg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Bovine Liver lysate;
Primary Ab: 1μg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 1μg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Serum
Primary Ab: 1μg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Bovine Liver lysate; Lane2: Bovine Serum; Lane3: Bovine Kidney lysate; Lane4: Porcine Serum;<br/>Primary Ab: 0.1μg/ml Rabbit Anti-Bovine RBP4 Antibody<br/>Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)RBP4的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別RBP4。
用法
Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA929Bo01 | 視黃醇結(jié)合蛋白4(RBP4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA929Bo02 | 視黃醇結(jié)合蛋白4(RBP4)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA929Bo02 | 視黃醇結(jié)合蛋白4(RBP4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA929Bo01 | 視黃醇結(jié)合蛋白4(RBP4)多克隆抗體 | WB,IHC |
| PAA929Bo02 | 視黃醇結(jié)合蛋白4(RBP4)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA929Bo03 | 視黃醇結(jié)合蛋白4(RBP4)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA929Bo81 | 視黃醇結(jié)合蛋白4(RBP4)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAA929Bo71 | 視黃醇結(jié)合蛋白4(RBP4)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA929Bo21 | 視黃醇結(jié)合蛋白4(RBP4)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA929Bo | 視黃醇結(jié)合蛋白4(RBP4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA929Bo | 視黃醇結(jié)合蛋白4(RBP4)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA929Bo01 | 視黃醇結(jié)合蛋白4(RBP4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Diabetes Technology & Therapeutics | Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 [Liebert: 20100078] |
| Journal of Pharmaceutical and Biomedical Analysis | Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2[PubMed: 21130593] |
| Evid Based Complement Alternat Med | Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in Rats[PubMed: PMC3368504] |
| Journal of Endocrinology | Combined galanin with insulin improves insulin sensitivity of diabetic rat muscles[Pubmed:24501381] |
| J Endocrinol | Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats[Pubmed:25240061] |
| Peptides | Effect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic rats[Pubmed:25445608] |
| Obesity Surgery | Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats[Pubmed:25697126] |
| Clin Exp Nephrol | Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome[PubMed: 26351173] |
| Experimental and Therapeutic Medicine | Expression of retinol binding protein 4 and nuclear factor-??B in diabetic rats with atherosclerosis and the intervention effect of pioglitazone[Pubmed:27446311] |
| Experimental and Therapeutic Medicine | Expression?of?retinol?binding?protein?4?and?nuclear?factor-κB in?diabetic?rats?with?atherosclerosisand the?intervention?effect?of?pioglitazone.[pubmed:27446311] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis[S0021967317311858] |
| BMC Cancer | Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis[Pubmed:29386009] |
| Nutrition &?Diabetes | Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans[Pubmed:29335416] |
| Molecular?Medicine?Reports | RBP4 regulates trophoblastic cell proliferation and invasion via the PI3K/AKT signaling pathway[Pubmed:30015949] |
| Relationship between retinol and risk of diabetic retinopathy: a case-control study[] | |
| Scientific Reports | Retinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattle[Pubmed: 31147589] |
| Potassium supplementation blunts the effects of high salt intake on serum retinol‐binding protein 4 levels in healthy individuals[] | |
| Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma[] | |
| Eur J Nutr | The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren[33575861] |
| Eur J Pediatr | Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren[33834274] |
| Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats[33833309] |
| Int Arch Occup Environ Health | Biomarkers of cadmium exposure and renal function in estuarine adult villagers[34773507] |