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視黃醇結合蛋白4(RBP4)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Retinol Binding Protein 4 (RBP4) ,etc. by FLIA (Flow Luminescence Immunoassay)

PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma

（注：單次混測多因子不超過8個指標 ）

  • 視黃醇結合蛋白4(RBP4)等多因子檢測試劑盒(流式熒光發(fā)光法)產品包裝(模擬)
  • 視黃醇結合蛋白4(RBP4)等多因子檢測試劑盒(流式熒光發(fā)光法)產品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測視黃醇結合蛋白4(RBP4)等多因子檢測試劑盒(流式熒光發(fā)光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的視黃醇結合蛋白4(RBP4)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)96-10399
EDTA plasma(n=5)78-9087
heparin plasma(n=5)80-9284

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內加入適量的視黃醇結合蛋白4(RBP4)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中視黃醇結合蛋白4(RBP4)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)85-102%86-99%84-96%92-101%
EDTA plasma(n=5)89-99%95-102%87-101%86-97%
heparin plasma(n=5)87-105%96-104%91-105%98-105%

穩(wěn)定性

經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將視黃醇結合蛋白4(RBP4)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的視黃醇結合蛋白4(RBP4)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的視黃醇結合蛋白4(RBP4)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的視黃醇結合蛋白4(RBP4)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

相關產品

編號適用物種：Bos taurus; Bovine (Cattle，牛)應用(僅供研究使用，不用于臨床診斷！)
RPA929Bo01視黃醇結合蛋白4(RBP4)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA929Bo02視黃醇結合蛋白4(RBP4)活性蛋白Cell?culture;?Activity?Assays.
RPA929Bo02視黃醇結合蛋白4(RBP4)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA929Bo01視黃醇結合蛋白4(RBP4)多克隆抗體WB; IHC
PAA929Bo02視黃醇結合蛋白4(RBP4)多克隆抗體WB; IHC; ICC; IP.
PAA929Bo03視黃醇結合蛋白4(RBP4)多克隆抗體WB
LAA929Bo81視黃醇結合蛋白4(RBP4)多克隆抗體(異硫氰酸熒光素標記)WB; IHC; ICC; IF.
LAA929Bo71視黃醇結合蛋白4(RBP4)多克隆抗體(生物素標記)IHC
MAA929Bo21視黃醇結合蛋白4(RBP4)單克隆抗體WB
SEA929Bo視黃醇結合蛋白4(RBP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA929Bo視黃醇結合蛋白4(RBP4)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA929Bo01視黃醇結合蛋白4(RBP4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志參考文獻
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Journal of Pharmaceutical and Biomedical AnalysisEffect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2[PubMed: 21130593]
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PeptidesEffect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic rats[Pubmed:25445608]
Obesity SurgeryLong-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats[Pubmed:25697126]
Clin Exp NephrolDifferentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome[PubMed: 26351173]
Experimental and Therapeutic MedicineExpression of retinol binding protein 4 and nuclear factor-??B in diabetic rats with atherosclerosis and the intervention effect of pioglitazone[Pubmed:27446311]
Experimental and Therapeutic MedicineExpression?of?retinol?binding?protein?4?and?nuclear?factor-κB in?diabetic?rats?with?atherosclerosisand the?intervention?effect?of?pioglitazone.[pubmed:27446311]
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BMC CancerScreening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis[Pubmed:29386009]
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Molecular?Medicine?ReportsRBP4 regulates trophoblastic cell proliferation and invasion via the PI3K/AKT signaling pathway[Pubmed:30015949]
Relationship between retinol and risk of diabetic retinopathy: a case-control study[]
Scientific ReportsRetinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattle[Pubmed: 31147589]
Potassium supplementation blunts the effects of high salt intake on serum retinol‐binding protein 4 levels in healthy individuals[]
Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma[]
Eur J NutrThe dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren[33575861]
Eur J PediatrPro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren[33834274]
Sci RepIleal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats[33833309]
Int Arch Occup Environ HealthBiomarkers of cadmium exposure and renal function in estuarine adult villagers[34773507]