聚集蛋白聚糖(AGC)檢測試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Aggrecan (AGC)
ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1
- 編號SCB908Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時長2h, 40min
- 檢測范圍41.2-30,000pg/mL
- 靈敏度最小可檢測劑量小于等于16.9pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實(shí)驗(yàn)結(jié)果圖
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測聚集蛋白聚糖(AGC)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的聚集蛋白聚糖(AGC)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 91-99 | 96 |
| EDTA plasma(n=5) | 82-89 | 86 |
| heparin plasma(n=5) | 92-101 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的聚集蛋白聚糖(AGC)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中聚集蛋白聚糖(AGC)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-99% | 98-105% | 93-101% | 87-95% |
| EDTA plasma(n=5) | 80-104% | 79-102% | 94-101% | 92-105% |
| heparin plasma(n=5) | 91-102% | 81-101% | 79-89% | 78-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實(shí)驗(yàn)原理
將聚集蛋白聚糖(AGC)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的聚集蛋白聚糖(AGC)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的聚集蛋白聚糖(AGC)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強(qiáng)度和樣品中的聚集蛋白聚糖(AGC)呈正相關(guān)。用化學(xué)發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB908Hu02 | 聚集蛋白聚糖(AGC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB908Hu01 | 聚集蛋白聚糖(AGC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB908Hu03 | 聚集蛋白聚糖(AGC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB908Hu02 | 聚集蛋白聚糖(AGC)多克隆抗體 | WB; IHC |
| PAB908Hu03 | 聚集蛋白聚糖(AGC)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB908Hu01 | 聚集蛋白聚糖(AGC)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB908Hu81 | 聚集蛋白聚糖(AGC)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB908Hu71 | 聚集蛋白聚糖(AGC)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB908Hu21 | 聚集蛋白聚糖(AGC)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB908Hu22 | 聚集蛋白聚糖(AGC)單克隆抗體 | WB |
| SEB908Hu | 聚集蛋白聚糖(AGC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB908Hu | 聚集蛋白聚糖(AGC)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB908Hu | 聚集蛋白聚糖(AGC)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB908Hu01 | 聚集蛋白聚糖(AGC)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] |
| Stem Cell Research & Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly.[Pubmed: 24739658] |
| Materials Science and Engineering: C | Co-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of composition[ScienceDirect: S0928493113006656] |
| PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media[Pubmed:Pmc4130592] |
| Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes[Pubmed:25333747] |
| Macromolecular Bioscience | Highly Porous Gelatin Reinforced 4D Scaffolds for Articular Cartilage Regeneration[PubMed: 25787871] |
| Osteoarthritis and Cartilage | Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized Cavia (Guinea pig )inea pigs[Pubmed:26802547] |
| Cell Proliferation | Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes[Pubmed:27090063] |
| bmc musculoskeletal disorders | Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis.[pubmed:28187731] |
| Journal of Clinical Medicine | Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors[Pubmed: 32392807] |
| Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs[] | |
| researchsquare | Aggrecan-a new biomarker for acute type A aortic dissection[33990642] |