聚集蛋白聚糖(AGC)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Aggrecan (AGC)
ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1
- 編號(hào)KSB908Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍1.56-100ng/mL
- 可適靈敏度0.59ng/mL
- 應(yīng)用Main materials for "Do It(ELISA Kit) Yourself"
- 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 7182¥ 11970¥ 21546¥ 41895¥ 71820
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)聚集蛋白聚糖(AGC)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB908Hu02 | 聚集蛋白聚糖(AGC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB908Hu01 | 聚集蛋白聚糖(AGC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB908Hu03 | 聚集蛋白聚糖(AGC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB908Hu02 | 聚集蛋白聚糖(AGC)多克隆抗體 | WB; IHC |
| PAB908Hu03 | 聚集蛋白聚糖(AGC)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB908Hu01 | 聚集蛋白聚糖(AGC)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB908Hu81 | 聚集蛋白聚糖(AGC)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB908Hu71 | 聚集蛋白聚糖(AGC)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB908Hu21 | 聚集蛋白聚糖(AGC)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB908Hu22 | 聚集蛋白聚糖(AGC)單克隆抗體 | WB |
| SEB908Hu | 聚集蛋白聚糖(AGC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB908Hu | 聚集蛋白聚糖(AGC)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB908Hu | 聚集蛋白聚糖(AGC)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB908Hu01 | 聚集蛋白聚糖(AGC)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Experimental Biology and Medicine | Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells[Rsmjournals: Source] |
| Age( dordr). | In vitro tenocyte metabolism in aging and oestrogen deficiency[Springer: Source] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] |
| Stem Cell Research & Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly.[Pubmed: 24739658] |
| Materials Science and Engineering: C | Co-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of composition[ScienceDirect: S0928493113006656] |
| PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media[Pubmed:Pmc4130592] |
| Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes[Pubmed:25333747] |
| Macromolecular Bioscience | Highly Porous Gelatin Reinforced 4D Scaffolds for Articular Cartilage Regeneration[PubMed: 25787871] |
| Osteoarthritis and Cartilage | Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized Cavia (Guinea pig )inea pigs[Pubmed:26802547] |
| Cell Proliferation | Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes[Pubmed:27090063] |
| bmc musculoskeletal disorders | Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis.[pubmed:28187731] |
| Journal of Clinical Medicine | Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors[Pubmed: 32392807] |
| Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs[] | |
| researchsquare | Aggrecan-a new biomarker for acute type A aortic dissection[33990642] |