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無翅型MMTV整合位點家族成員3A(WNT3A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)

  • 無翅型MMTV整合位點家族成員3A(WNT3A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • 無翅型MMTV整合位點家族成員3A(WNT3A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質量體系認證

特異性

試劑盒中的抗體對無翅型MMTV整合位點家族成員3A(WNT3A)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

參考文獻

雜志參考文獻
Medical OncologyWls promotes the proliferation of breast cancer cells via Wnt signaling[Pubmed:25801232]
J Histochem CytochemWnt3a Mediates the Inhibitory Effect of Hyperoxia on the Transdifferentiation of AECIIs to AECIs[PubMed: 26209081]
PloS oneGelatin device for the delivery of growth factors involved in endochondral ossification[pubmed:28380024]
Life SciencesHepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways.[pubmed:28754618]
Oxidative Medicine and Cellular LongevitySecreted Frizzled-Related Protein-2 Inhibits Doxorubicin Induced Apoptosis Mediated Through the Akt-mTOR Pathway in Soleus Muscle[Pubmed:30151071]
The Showa University Journal of Medical SciencesEribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer[Doi: 10.15369/sujms.30.359]
Journal of CancerOncogenic Wnt3a: A Candidate Specific Marker and Novel Molecular Target for Hepatocellular Carcinoma[Pubmed: 31737122]
CANCER LETTERSKnock down of BMSC-derived Wnt3a or its antagonist analogs attenuate colorectal carcinogenesis induced by chronic Fusobacterium nucleatum infection[Pubmed: 32920199]
BiomaterialsAn improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties[34823885]