無翅型MMTV整合位點家族成員3A(WNT3A)單克隆抗體
Monoclonal Antibody to Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)
- 編號MAP155Hu21
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C2
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原RPP155Hu01-無翅型MMTV整合位點家族成員3A(WNT3A)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種-
- 應用WB; IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 731¥ 1705¥ 2436¥ 6090¥ 24360
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Sample: Human Placenta Tissue; Primary Ab: 30μg/ml Mouse Anti-Human WNT3A Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Small intestine Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human WNT3A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Figure. Western Blot; Sample: Recombinant WNT3A, Human.
Western Blot; Sample: MCF7 cell lysate<br/>Primary Ab: 3μg/ml Mouse Anti-Human WNT3A Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對WNT3A的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別WNT3A。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPP155Hu01 | 無翅型MMTV整合位點家族成員3A(WNT3A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAP155Hu01 | 無翅型MMTV整合位點家族成員3A(WNT3A)多克隆抗體 | WB; IHC; ICC; IP. |
| MAP155Hu21 | 無翅型MMTV整合位點家族成員3A(WNT3A)單克隆抗體 | WB; IHC |
| MAP155Hu22 | 無翅型MMTV整合位點家族成員3A(WNT3A)單克隆抗體 | IHC |
| MAP155Hu23 | 無翅型MMTV整合位點家族成員3A(WNT3A)單克隆抗體 | IHC |
| MAP155Hu24 | 無翅型MMTV整合位點家族成員3A(WNT3A)單克隆抗體 | IHC |
| MAP155Hu25 | 無翅型MMTV整合位點家族成員3A(WNT3A)單克隆抗體 | IHC |
| SEP155Hu | 無翅型MMTV整合位點家族成員3A(WNT3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEP155Hu | 無翅型MMTV整合位點家族成員3A(WNT3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMP155Hu | 無翅型MMTV整合位點家族成員3A(WNT3A)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSP155Hu01 | 無翅型MMTV整合位點家族成員3A(WNT3A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Medical Oncology | Wls promotes the proliferation of breast cancer cells via Wnt signaling[Pubmed:25801232] |
| J Histochem Cytochem | Wnt3a Mediates the Inhibitory Effect of Hyperoxia on the Transdifferentiation of AECIIs to AECIs[PubMed: 26209081] |
| PloS one | Gelatin device for the delivery of growth factors involved in endochondral ossification[pubmed:28380024] |
| Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways.[pubmed:28754618] |
| Oxidative Medicine and Cellular Longevity | Secreted Frizzled-Related Protein-2 Inhibits Doxorubicin Induced Apoptosis Mediated Through the Akt-mTOR Pathway in Soleus Muscle[Pubmed:30151071] |
| The Showa University Journal of Medical Sciences | Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer[Doi: 10.15369/sujms.30.359] |
| Journal of Cancer | Oncogenic Wnt3a: A Candidate Specific Marker and Novel Molecular Target for Hepatocellular Carcinoma[Pubmed: 31737122] |
| CANCER LETTERS | Knock down of BMSC-derived Wnt3a or its antagonist analogs attenuate colorectal carcinogenesis induced by chronic Fusobacterium nucleatum infection[Pubmed: 32920199] |
| Biomaterials | An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties[34823885] |